Cell Calcium. 2013 Oct;54(4):307-19. doi: 10.1016/j.ceca.2013.07.006. Epub 2013 Aug 14.

Activation of TRPC4beta by Galphai subunit increases Ca2+ selectivity and controls neurite morphogenesis in cultured hippocampal neuron.External 2231691f894ba696de1310221b0a0dbbb31a7251e75115c265587c3d9d5f507c

Jeon, J. P., Roh, S. E., Wie, J., Kim, J., Kim, H., Lee, K. P., Yang, D., Jeon, J. H., Cho, N. H., Kim, I. G., Kang, D. E., Kim, H. J., So, I.,
["Department of Physiology and Institute of Dermatological Science, Seoul National University College of Medicine, Seoul 110-799, Republic of Korea."]
The ubiquitous transient receptor potential canonical (TRPC) channels function as non-selective, Ca(2+)-permeable channels. TRPC channels are activated by stimulation of Galphaq-PLC-coupled receptors. Here, we report that TRPC4/TRPC5 can be activated by Galphai. We studied the essential role of Galphai subunits in TRPC4 activation and investigated changes in ion selectivity and pore dilation of the TRPC4 channel elicited by the Galphai2 subunit. Activation of TRPC4 by Galphai2 increased Ca2+ permeability and Ca2+ influx through TRPC4 channels. Co-expression of the muscarinic receptor (M2) and TRPC4 in HEK293 cells induced TRPC4-mediated Ca2+ influx. Moreover, both TRPC4beta and the TRPC4beta-Galphai2 signaling complex induced inhibition of neurite growth and arborization in cultured hippocampal neurons. Cells treated with KN-93, a CaMKII inhibitor, prevented TRPC4- and TRPC4-Galphai2(Q205L)-mediated inhibition of neurite branching and growth. These findings indicate an essential role of Galphai proteins in TRPC4 activation and extend our knowledge of the functional role of TRPC4 in hippocampal neurons.
PMID: 24011658External 2231691f894ba696de1310221b0a0dbbb31a7251e75115c265587c3d9d5f507c
Validation: In vivo validation Toggle 893349bafcc528f8346c51dc3420151d67b0126b2c122dd1017121c03fa0f69b
  Assay with endogenous proteins Assay with overexpressed proteins Reference
Cell or tissue Cell or tissue TRP channel construct Interactor construct
TRP channel Interactor Method Species Region Species Region
TRPC4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Gơi2 Co-immunoprecipitation HEK293 Mouse Full-length Human Full-length 24011658
(Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
TRP / Interactor

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