Neurochem Int. 2010 Jan;56(2):363-6. doi: 10.1016/j.neuint.2009.11.009. Epub 2009 Nov 20.
The interactions of the C-terminal region of the TRPC6 channel with calmodulin.
Friedlova, E., Grycova, L., Holakovska, B., Silhan, J., Janouskova, H., Sulc, M., Obsilova, V., Obsil, T., Teisinger, J.,
["Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic."]
["Institute of Physiology, Academy of Sciences of the Czech Republic, Prague, Czech Republic."]
The transient receptor potential channel TRPC6 is a non-selective cation channel which modulates the calcium level in eukaryotic cells (including sensory receptor cells) in response to external signals. Calmodulin (CaM) is a ubiquitously expressed Ca(2+) binding protein that is an important mediator of Ca(2+)-dependent regulation of the TRPC6 channel. One CaM binding site was identified within the C-tail of TRPC6. The aim of this study is to map in detail the CaM and inositol (1,4,5)-triphosphate receptor binding (CIRB) domain in the C-terminal region of mouse TRPC6 that is capable of interacting with CaM using in vitro binding assays. Besides the set of positively charged amino acid residues Arg852, Lys856, Arg864, Lys859/Arg860, a hydrophobic Ile857, at the position 1 in 1-5-10 motif, was located and the effect of replacing it with a neutral residue was tested using fluorescence anisotropy measurement. Participation of Ile857 could indicate a strong role of this conserved CaM binding motif.
PMID: 19932145
 Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
Characterization
|
||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | |||||||
| TRP channel | Interactor | |||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
| TRPC6 |
|
Calmodulin | Fluorescence probe labeling | 320 nM | 19932145 | |||||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)