J Biol Chem. 2007 Jun 1;282(22):16631-43. Epub 2007 Apr 6.
Genetic evidence supporting caveolae microdomain regulation of calcium entry in endothelial cells.
Murata, T., Lin, M. I., Stan, R. V., Bauer, P. M., Yu, J., Sessa, W. C.,
["Department of Pharmacology and Program in Vascular Cell Signaling and Therapeutics, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, Connecticut 06536, USA."]
["Department of Pharmacology and Program in Vascular Cell Signaling and Therapeutics, Boyer Center for Molecular Medicine, Yale University School of Medicine, New Haven, Connecticut 06536, USA."]
Various cellular signals initiate calcium entry into cells, and there is evidence that lipid rafts and caveolae may concentrate proteins that regulate transmembrane calcium fluxes. Here, using mice deficient in caveolin-1 (Cav-1) and Cav-1 knock-out reconstituted with endothelium-specific Cav-1, we show that Cav-1 is essential for calcium entry in endothelial cells and governs the localization and protein-protein interactions between transient receptor channels C4 and C1. Thus, Cav-1 is required for calcium entry in vascular endothelial cells and perhaps other specialized cell types containing caveolae.
PMID: 17416589
 Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
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Screening
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| Experimental screening | Non-experimental screening | Reference | ||||||||
| TRP channel construct | Interactor source | |||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | |||
| TRPC4 |
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Caveolin-1 | Inference | Prediction | 17416589 | |||||
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
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Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
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top
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| Validation: In vitro validation
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| Assay with recombinant proteins | Reference | |||||||||
| TRP channel construct | Interactor construct | |||||||||
| TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | ||
| TRPC4 |
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Caveolin-1 | Fusion protein-pull down assay | Not used | Mouse lung endothelium cell lysates | Not specified | Full-length | E. coli | 17416589 | |
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
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Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
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top
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| Validation: In vivo validation
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| Assay with endogenous proteins | Assay with overexpressed proteins | Reference | ||||||||
| Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | |||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
| TRPC1 |
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TRPC4 | Co-immunoprecipitation | Mouse lung endothelium cell | 17416589 | |||||
| TRPC4 |
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Caveolin-1 | Co-immunofluorescence staining | Mouse lung endothelium cell | 17416589 | |||||
| TRPC4 |
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Caveolin-1 | Co-immunoprecipitation | Mouse lung endothelium cell | 17416589 | |||||
| TRPC4 |
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TRPC1 | Co-immunoprecipitation | Mouse lung endothelium cell | 17416589 | |||||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
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| Characterization
|
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|---|---|---|---|---|---|---|---|---|---|---|
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | |||||||
| TRP channel | Interactor | |||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
| TRPC4 |
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Caveolin-1 | Fusion protein-pull down assay | Mouse | Not determined | Not specified | 61-101 | 17416589 | ||
| TRPC4 |
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Caveolin-1 | Fusion protein-pull down assay | Mouse | Not determined | Not specified | 135-178 | 17416589 | ||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)