J Neurosci. 2006 Mar 1;26(9):2403-12.
TRPV1 recapitulates native capsaicin receptor in sensory neurons in association with Fas-associated factor 1.
Kim, S., Kang, C., Shin, C. Y., Hwang, S. W., Yang, Y. D., Shim, W. S., Park, M. Y., Kim, E., Kim, M., Kim, B. M., Cho, H., Shin, Y., Oh, U.,
["Sensory Research Center, College of Pharmacy, Seoul National University, Seoul 151-742, Korea."]
["Sensory Research Center, College of Pharmacy, Seoul National University, Seoul 151-742, Korea."]
TRPV1, a cloned capsaicin receptor, is a molecular sensor for detecting adverse stimuli and a key element for inflammatory nociception and represents biophysical properties of native channel. However, there seems to be a marked difference between TRPV1 and native capsaicin receptors in the pharmacological response profiles to vanilloids or acid. One plausible explanation for this overt discrepancy is the presence of regulatory proteins associated with TRPV1. Here, we identify Fas-associated factor 1 (FAF1) as a regulatory factor, which is coexpressed with and binds to TRPV1 in sensory neurons. When expressed heterologously, FAF1 reduces the responses of TRPV1 to capsaicin, acid, and heat, to the pharmacological level of native capsaicin receptor in sensory neurons. Furthermore, silencing FAF1 by RNA interference augments capsaicin-sensitive current in native sensory neurons. We therefore conclude that FAF1 forms an integral component of the vanilloid receptor complex and that it constitutively modulates the sensitivity of TRPV1 to various noxious stimuli in sensory neurons.
PMID: 16510717

![]() ![]() ![]() ![]() ![]() |
![]() |
Screening
![]() |
||||||||||
---|---|---|---|---|---|---|---|---|---|---|
Experimental screening | Non-experimental screening | Reference | ||||||||
TRP channel construct | Interactor source | |||||||||
TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | |||
TRPV1 |
![]() |
FAF1 | Yeast two-hybrid | Rat | 243-440 | Rat | Sensory neuron | cDNA expression library | 16510717 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

![]() ![]() ![]() ![]() ![]() |
![]() |
Validation: In vitro validation
![]() |
||||||||||
---|---|---|---|---|---|---|---|---|---|---|
Assay with recombinant proteins | Reference | |||||||||
TRP channel construct | Interactor construct | |||||||||
TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | ||
TRPV1 |
![]() |
FAF1 | Fusion protein-pull down assay | Rat | 1-440 | E. coli | Rat | Full-length | E. coli | 16510717 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

![]() ![]() ![]() ![]() ![]() |
![]() |
Validation: In vivo validation
![]() |
||||||||||
---|---|---|---|---|---|---|---|---|---|---|
Assay with endogenous proteins | Assay with overexpressed proteins | Reference | ||||||||
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | |||||||
TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
TRPV1 |
![]() |
FAF1 | Co-immunoprecipitation | Rat dorsal root ganglion cell | 16510717 | |||||
TRPV1 |
![]() |
FAF1 | Co-immunoprecipitation | HEK293T | Rat | Full-length | Rat | Full-length | 16510717 | |
TRPV1 |
![]() |
FAF1 | Co-immunofluorescence staining | Rat dorsal root ganglion cell | 16510717 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

![]() ![]() ![]() ![]() ![]() |
![]() |
Characterization
![]() |
||||||||||
---|---|---|---|---|---|---|---|---|---|---|
Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | |||||||
TRP channel | Interactor | |||||||||
TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
TRPV1 |
![]() |
FAF1 | Fusion protein-pull down assay | Rat | 243-440 | Rat | 110-169 | 16510717 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

![]() ![]() ![]() ![]() ![]() |
![]() |
Functional consequence
![]() |
||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
TRP channel | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference | ||||||
TRPV1 |
![]() |
FAF1 | Calcium measurement | Inhibition | 16510717 | |||||||
TRPV1 |
![]() |
FAF1 | Patch clamp | Inhibition | 16510717 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
