J Exp Med. 2004 Jul 19;200(2):137-47. Epub 2004 Jul 12.
A TRPV2-PKA signaling module for transduction of physical stimuli in mast cells.
Stokes, A. J., Shimoda, L. M., Koblan-Huberson, M., Adra, C. N., Turner, H.,
["Center for Biomedical Research, 1301 Punchbowl St., University Tower 8, Honolulu, HI 96813, USA."]
["Center for Biomedical Research, 1301 Punchbowl St., University Tower 8, Honolulu, HI 96813, USA."]
Cutaneous mast cell responses to physical (thermal, mechanical, or osmotic) stimuli underlie the pathology of physical urticarias. In vitro experiments suggest that mast cells respond directly to these stimuli, implying that a signaling mechanism couples functional responses to physical inputs in mast cells. We asked whether transient receptor potential (vanilloid) (TRPV) cation channels were present and functionally coupled to signaling pathways in mast cells, since expression of this channel subfamily confers sensitivity to thermal, osmotic, and pressure inputs. Transcripts for a range of TRPVs were detected in mast cells, and we report the expression, surface localization, and oligomerization of TRPV2 protein subunits in these cells. We describe the functional coupling of TRPV2 protein to calcium fluxes and proinflammatory degranulation events in mast cells. In addition, we describe a novel protein kinase A (PKA)-dependent signaling module, containing PKA and a putative A kinase adapter protein, Acyl CoA binding domain protein (ACBD)3, that interacts with TRPV2 in mast cells. We propose that regulated phosphorylation by PKA may be a common pathway for TRPV modulation.
PMID: 15249591

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Screening
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Experimental screening | Non-experimental screening | Reference | ||||||||
TRP channel construct | Interactor source | |||||||||
TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | |||
TRPV2 |
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ACBD3 | Inference | Prediction | 15249591 | |||||
TRPV2 |
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PKA | Inference | Prediction | 15249591 | |||||
TRPV2 |
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PKARIIơ | Inference | Prediction | 15249591 |
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Validation: In vivo validation
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Assay with endogenous proteins | Assay with overexpressed proteins | Reference | ||||||||
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | |||||||
TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
TRPV2 |
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ACBD3 | Co-immunoprecipitation | RBL-2H3 | 15249591 | |||||
TRPV2 |
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PKARIIơ | Co-immunoprecipitation | RBL-2H3 | 15249591 |
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Functional consequence
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TRP channel | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference | ||||||
TRPV2 |
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PKA | In vitro PTM assay | Phosphorylation | 15249591 |
(
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
