Neuron. 2001 Mar;29(3):645-55.
TRPC1 and TRPC5 form a novel cation channel in mammalian brain.
Strubing, C., Krapivinsky, G., Krapivinsky, L., Clapham, D. E.,
["Howard Hughes Medical Institute, Cardiovascular Research, Children's Hospital, 320 Longwood Avenue, Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA."]
["Howard Hughes Medical Institute, Cardiovascular Research, Children's Hospital, 320 Longwood Avenue, Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA."]
TRP proteins are cation channels responding to receptor-dependent activation of phospholipase C. Mammalian (TRPC) channels can form hetero-oligomeric channels in vitro, but native TRPC channel complexes have not been identified to date. We demonstrate here that TRPC1 and TRPC5 are subunits of a heteromeric neuronal channel. Both TRPC proteins have overlapping distributions in the hippocampus. Coexpression of TRPC1 and TRPC5 in HEK293 cells resulted in a novel nonselective cation channel with a voltage dependence similar to NMDA receptor channels, but unlike that of any reported TRPC channel. TRPC1/TRPC5 heteromers were activated by G(q)-coupled receptors but not by depletion of intracellular Ca(2+) stores. In contrast to the more common view of the TRP family as comprising store-operated channels, we propose that many TRPC heteromers form diverse receptor-regulated nonselective cation channels in the mammalian brain.
PMID: 11301024

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Screening
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Experimental screening | Non-experimental screening | Reference | ||||||||
TRP channel construct | Interactor source | |||||||||
TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | |||
TRPC1 |
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TRPC4 | Inference | Prediction | 11301024 | |||||
TRPC1 |
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TRPC5 | Inference | Prediction | 11301024 | |||||
TRPC4 |
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TRPC1 | Inference | Prediction | 11301024 | |||||
TRPC5 |
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TRPC1 | Inference | Prediction | 11301024 |
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Validation: In vivo validation
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Assay with endogenous proteins | Assay with overexpressed proteins | Reference | ||||||||
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | |||||||
TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
TRPC1 |
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TRPC4 | Co-immunoprecipitation | Rat brain microsome | 11301024 | |||||
TRPC1 |
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TRPC5 | Co-immunoprecipitation | Rat brain microsome | 11301024 | |||||
TRPC1 |
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TRPC5 | Co-immunoprecipitation | HEK293 | Human | Full-length | Mouse | Full-length | 11301024 | |
TRPC4 |
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TRPC1 | Co-immunoprecipitation | Rat brain microsome | 11301024 | |||||
TRPC5 |
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TRPC1 | Co-immunoprecipitation | Rat brain microsome | 11301024 | |||||
TRPC5 |
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TRPC1 | Co-immunoprecipitation | HEK293 | Mouse | Full-length | Human | Full-length | 11301024 |
(
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Functional consequence
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TRP channel | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference | ||||||
TRPC1 |
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TRPC4 | Patch clamp | New channel creation | 11301024 | |||||||
TRPC1 |
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TRPC5 | Patch clamp | New channel creation | 11301024 | |||||||
TRPC4 |
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TRPC1 | Patch clamp | New channel creation | 11301024 | |||||||
TRPC5 |
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TRPC1 | Patch clamp | New channel creation | 11301024 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
