Mol Cell. 1999 Sep;4(3):423-9.
The N-terminal domain of the IP3 receptor gates store-operated hTrp3 channels.
Kiselyov, K., Mignery, G. A., Zhu, M. X., Muallem, S.,
["Department of Physiology, University of Texas Southwestern Medical Center, Dallas 75235, USA."]
["Department of Physiology, University of Texas Southwestern Medical Center, Dallas 75235, USA."]
In the present work, we studied the interaction and effect of several IP3 receptor (IP3R) constructs on the gating of the store-operated (SOC) hTrp3 channel. Full-length IP3R coupled to silent hTrp3 channels in intact cells but did not activate them until stores were depleted of Ca2+. By contrast, constructs containing the IP3-binding domain activated silent hTrp3 channels in unstimulated cells and restored gating of hTrp3 by IP3 in excised plasma membrane patches. We conclude that the N-terminal domain of the IP3R functions as a gate and is sufficient for activation of SOCs. The sensing and transduction domains of the IP3R are required to maintain SOCs in an inactive state.
PMID: 10518223

![]() ![]() ![]() ![]() ![]() |
![]() |
Screening
![]() |
||||||||||
---|---|---|---|---|---|---|---|---|---|---|
Experimental screening | Non-experimental screening | Reference | ||||||||
TRP channel construct | Interactor source | |||||||||
TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | |||
TRPC3 |
![]() |
IP3R1 | Inference | Prediction | 10518223 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

![]() ![]() ![]() ![]() ![]() |
![]() |
Validation: In vivo validation
![]() |
||||||||||
---|---|---|---|---|---|---|---|---|---|---|
Assay with endogenous proteins | Assay with overexpressed proteins | Reference | ||||||||
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | |||||||
TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
TRPC3 |
![]() |
IP3R1 | Co-immunoprecipitation | HEK293 | Human | Full-length | Rat | Full-length | 10518223 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

![]() ![]() ![]() ![]() ![]() |
![]() |
Characterization
![]() |
||||||||||
---|---|---|---|---|---|---|---|---|---|---|
Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | |||||||
TRP channel | Interactor | |||||||||
TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
TRPC3 |
![]() |
IP3R1 | Co-immunoprecipitation | Human | Not determined | Rat | IP3 binding domain | 10518223 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

![]() ![]() ![]() ![]() ![]() |
![]() |
Functional consequence
![]() |
||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
TRP channel | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference | ||||||
TRPC3 |
![]() |
IP3R1 | Calcium measurement | Activation | 10518223 | |||||||
TRPC3 |
![]() |
IP3R1 | Patch clamp | Activation | 10518223 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
