p97/VCP / TRP channels

Name: Transitional endoplasmic reticulum ATPase (p97/VCP)
Official Symbol: VCP provided by HGNC
Function:
Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. Regulates E3 ubiquitin-protein ligase activity of RNF19A (By similarity). Component of the VCP/p97-AMFR/gp78 complex that participates in the final step of the sterol-mediated ubiquitination and endoplasmic reticulum-associated degradation (ERAD) of HMGCR. Also involved in DNA damage response: recruited to double-strand breaks (DSBs) sites in a RNF8-and RNF168-dependent manner and promotes the recruitment of TP53BP1 at DNA damage sites. Recruited to stalled replication forks by SPRTN: may act by mediating extraction of DNA polymerase eta (POLH) to prevent excessive translesion DNA synthesis and limit the incidence of mutations induced by DNA damage.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).
Species External DB Questionmark c7dcdfe7852228a510704064c33a0706d8c0e915bceea718517e77f7950c2984
Entrez Gene UniprotKB DIP IntAct MINT BioGRID STRING IUPHAR-DB KEGG OMIM
Human
Seq 055e86e69a1b9fa3c345494da0168454ea5fcb72eb0f6a154a55bbbbbcb46d2f
7415External P55072External DIP-33543NExternal P55072External MINT-272884External 113258External P55072External   hsa:7415External 601023External
Mouse
Seq 055e86e69a1b9fa3c345494da0168454ea5fcb72eb0f6a154a55bbbbbcb46d2f
269523External Q01853External DIP-29796NExternal Q01853External MINT-220770External 234661External Q01853External   mmu:269523External  
Rat
Seq 055e86e69a1b9fa3c345494da0168454ea5fcb72eb0f6a154a55bbbbbcb46d2f
116643External P46462External DIP-32904NExternal P46462External MINT-1954391External 250528External P46462External   rno:116643External  
PPI pairs:
Biological Process:
  • GO:0006919 External 2231691f894ba696de1310221b0a0dbbb31a7251e75115c265587c3d9d5f507c : activation of cysteine-type endopeptidase activity involved in apoptotic process
  • GO:0070842 External 2231691f894ba696de1310221b0a0dbbb31a7251e75115c265587c3d9d5f507c : aggresome assembly
  • GO:0006302 External 2231691f894ba696de1310221b0a0dbbb31a7251e75115c265587c3d9d5f507c : double-strand break repair
  • GO:0030968 External 2231691f894ba696de1310221b0a0dbbb31a7251e75115c265587c3d9d5f507c : endoplasmic reticulum unfolded protein response
  • GO:0006888 External 2231691f894ba696de1310221b0a0dbbb31a7251e75115c265587c3d9d5f507c : ER to Golgi vesicle-mediated transport
  • GO:0030433 External 2231691f894ba696de1310221b0a0dbbb31a7251e75115c265587c3d9d5f507c : ER-associated protein catabolic process
  • GO:0032436 External 2231691f894ba696de1310221b0a0dbbb31a7251e75115c265587c3d9d5f507c : positive regulation of proteasomal ubiquitin-dependent protein catabolic process
  • GO:0031334 External 2231691f894ba696de1310221b0a0dbbb31a7251e75115c265587c3d9d5f507c : positive regulation of protein complex assembly
  • GO:0051260 External 2231691f894ba696de1310221b0a0dbbb31a7251e75115c265587c3d9d5f507c : protein homooligomerization
  • GO:0018279 External 2231691f894ba696de1310221b0a0dbbb31a7251e75115c265587c3d9d5f507c : protein N-linked glycosylation via asparagine
  • GO:0016567 External 2231691f894ba696de1310221b0a0dbbb31a7251e75115c265587c3d9d5f507c : protein ubiquitination
  • GO:0030970 External 2231691f894ba696de1310221b0a0dbbb31a7251e75115c265587c3d9d5f507c : retrograde protein transport, ER to cytosol
  • GO:0019985 External 2231691f894ba696de1310221b0a0dbbb31a7251e75115c265587c3d9d5f507c : translesion synthesis
Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease:
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/
Screening Toggle 893349bafcc528f8346c51dc3420151d67b0126b2c122dd1017121c03fa0f69b
  Experimental screening Non-experimental screening Reference
TRP channel construct Interactor source
TRP channel Interactor Method Species Region Species Organ/tissue Sample type
TRPC3 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 p97/VCP Affinity purification-mass spectrometry Not used as a bait Rat Brain Crude membranes 18205297
TRPP1 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 p97/VCP Affinity purification-mass spectrometry Mouse Not specified Mouse Vascular smooth muscle MOVAS lysates 19879844
TRPP1 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 p97/VCP Inference Prediction 18178578
(Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Validation: In vivo validation Toggle 893349bafcc528f8346c51dc3420151d67b0126b2c122dd1017121c03fa0f69b
  Assay with endogenous proteins Assay with overexpressed proteins Reference
Cell or tissue Cell or tissue TRP channel construct Interactor construct
TRP channel Interactor Method Species Region Species Region
TRPP1 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 p97/VCP Co-immunofluorescence staining MDCK Human Full-length Not used 18178578
TRPP1 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 p97/VCP Co-immunoprecipitation Mouse Inner medullary collecting duct cell 18178578
(Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
TRP / Interactor

To prevent spam comments, we use reCAPTCHA. Please type correct words into the following box.