TRPV4 / interactors

Name: Transient receptor potential cation channel subfamily V member 4 (TRPV4)
Official Symbol: TRPV4 provided by HGNC
Function:
Non-selective calcium permeant cation channel probably involved in osmotic sensitivity and mechanosensitivity. Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification. Also activated by low pH, citrate and phorbol esters. Increase of intracellular Ca(2+) potentiates currents. Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism. Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers. Acts as a regulator of intracellular Ca(2+) in synoviocytes. Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt) Nucleic Acids Res. 40: D71-D75 (2012).
Species External DB Questionmark c7dcdfe7852228a510704064c33a0706d8c0e915bceea718517e77f7950c2984
Entrez Gene UniprotKB DIP IntAct MINT BioGRID STRING IUPHAR-DB KEGG OMIM
Human
Seq 055e86e69a1b9fa3c345494da0168454ea5fcb72eb0f6a154a55bbbbbcb46d2f
59341External Q9HBA0External DIP-35702NExternal Q9HBA0External MINT-4535111External 121883External Q9HBA0External 510External hsa:59341External 605427External
Mouse
Seq 055e86e69a1b9fa3c345494da0168454ea5fcb72eb0f6a154a55bbbbbcb46d2f
63873External Q9EPK8External DIP-44011NExternal   MINT-4050076External 211003External Q9EPK8External   mmu:63873External  
Rat
Seq 055e86e69a1b9fa3c345494da0168454ea5fcb72eb0f6a154a55bbbbbcb46d2f
66026External Q9ERZ8External DIP-59600NExternal     249353External Q9ERZ8External   rno:66026External  
Biological Process:
Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
Disease:
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine, Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/
Screening Toggle 893349bafcc528f8346c51dc3420151d67b0126b2c122dd1017121c03fa0f69b
  Experimental screening Non-experimental screening Reference
TRP channel construct Interactor source
TRP channel Interactor Method Species Region Species Organ/tissue Sample type
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 80K-H Speculation Prediction 19801576
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 AKAP5/AKAP150 Speculation Prediction 18701070
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 AQP-4 Inference Prediction 21262839
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 AQP-5 Inference Prediction 16571723
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 AT1aR Inference Prediction 20650893
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Bkca Inference Prediction 25139746
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Calmodulin Inference Prediction 12724311
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Caveolin-1 Inference Prediction 20705915
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 E-cadherin Inference Prediction 20413591
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 FYN Inference Prediction 12538589
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 ƣ-actin Inference Prediction 22101010
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Ƣ-actin Inference Prediction 19027987
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Ƣ-arrestin 1 Inference Prediction 20650893
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Ƣ-arrestin 2 Affinity purification-mass spectrometry Not used as a bait Human HEK293 lysates 17620599
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Ƣ-catenin Yeast two-hybrid Mouse N-terminus Human Keratinocyte cDNA library 20413591
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Ƣ-tubulin-5 Inference Prediction 22309793
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 HCK Inference Prediction 12538589
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 IP3R3 Inference Prediction 18378772
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 ITCH/AIP4 Inference Prediction 17110928
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 LCK Inference Prediction 12538589
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 LYN Inference Prediction 12538589
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 MAP7 Yeast two-hybrid Mouse 783-871 Mouse Kidney cDNA library 14517216
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Myosin-10 Affinity purification-mass spectrometry Mouse Full-length Human HEK293 Cell lystaes 22492541
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Myosin-HIIA Affinity purification-mass spectrometry Mouse Full-length Human HEK293 Cell lystaes 22492541
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 NHERF4 Inference Prediction 16565876
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 ơ2-integrin Inference Prediction 18234883
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 OS-9 Yeast two-hybrid Mouse 61-467 Human adult kidney cDNA library 17932042
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 PACS-1 Inference Prediction 15692563
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Pacsin1 Yeast two-hybrid Chicken N-terminus Chicken Inner ear cDNA library 16627472
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Pacsin2 Inference Prediction 16627472
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Pacsin3 Yeast two-hybrid Chicken N-terminus Chicken Inner ear cDNA library 16627472
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 RPS27A Affinity purification-mass spectrometry Mouse Full-length Human HEK293 Cell lystaes 22492541
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 SRC Inference Prediction 12538589
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 TMEM16A/ANO1 Inference Prediction 24509911
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 TRPC1 Inference Prediction 20705915
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 TRPP1 Inference Prediction 18695040
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 UBC Inference Prediction 17110928
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 YES Inference Prediction 12538589
(Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Validation: In vitro validation Toggle 893349bafcc528f8346c51dc3420151d67b0126b2c122dd1017121c03fa0f69b
  Assay with recombinant proteins Reference
TRP channel construct Interactor construct
TRP channel Interactor Method Species Region Expression system Species Region Expression system
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Calmodulin Fusion protein-pull down assay Human C-terminus E. coli Not specified Full-length Not specified 12724311
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Calmodulin Fusion protein-pull down assay Mouse Full-length HEK293 lysates Not specified Not specified In vitro translation 22492541
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Calmodulin Fusion protein-pull down assay Human 718-871 E. coli Not used HEK293T lysates 21837266
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 ƣ-actin Fusion protein-pull down assay Mouse Full-length E. coli Not used HEK293 lysates 22101010
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 MAP7 Fusion protein-pull down assay Mouse Full-length HEK293 lysates Mouse Full-length In vitro translation 14517216
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 NHERF4 Fusion protein-pull down assay Mouse C-terminus E. coli Mouse Full-length In vitro translation 16565876
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Tubulin Fusion protein-pull down assay Mouse Full-length E. coli Not used HEK293 lysates 22101010
(Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Validation: In vivo validation Toggle 893349bafcc528f8346c51dc3420151d67b0126b2c122dd1017121c03fa0f69b
  Assay with endogenous proteins Assay with overexpressed proteins Reference
Cell or tissue Cell or tissue TRP channel construct Interactor construct
TRP channel Interactor Method Species Region Species Region
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 80K-H Co-immunoprecipitation HEK293T Mouse Full-length Human Full-length 19801576
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 AKAP5/AKAP150 Co-immunofluorescence staining Rat cerebral artery 24778429
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 AKAP5/AKAP150 Co-immunoprecipitation HEK293 Human Full-length Not used 18701070
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 AQP-4 Co-immunofluorescence staining Adult rat brain section 21262839
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 AQP-4 Co-immunoprecipitation Adult rat brain lysates 21262839
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 AQP-4 Co-immunoprecipitation DI TNC1 Human Full-length Rat Full-length 21262839
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 AQP-5 Co-immunoprecipitation Mouse submandibular gland cell 16571723
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 AQP-5 Co-immunoprecipitation HSG cell 16571723
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 AT1aR Co-immunofluorescence staining Rat vascular smooth muscle cell Human Full-length Human Full-length 20650893
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 AT1aR Co-immunoprecipitation Rat vascular smooth muscle cell Not used Human Full-length 20650893
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 AT1aR Co-immunoprecipitation HEK293 Human Full-length Human Full-length 20650893
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Bkca Co-immunoprecipitation Porcine coronary artery 25139746
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Caveolin-1 Co-immunofluorescence staining HUVEC Human Full-length Mouse Full-length 20705915
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 E-cadherin Co-immunoprecipitation MDCK 20413591
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 E-cadherin Co-immunoprecipitation HEK 22374181
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 FYN Co-immunoprecipitation HEK293 Mouse Full-length Not used 12538589
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Ƣ-actin Co-immunofluorescence staining HaCaT Human Full-length Not used 19027987
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 ƣ-actin Co-immunoprecipitation HEK293 Mouse Full-length Not used 22101010
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Ƣ-actin Fluorescence resonance energy transfer CHO Human Full-length Human Full-length 19027987
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Ƣ-arrestin 1 Co-immunoprecipitation Rat vascular smooth muscle cell 20650893
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Ƣ-arrestin 1 Co-immunoprecipitation HEK293 Human Full-length Human Full-length 20650893
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Ƣ-catenin Co-immunoprecipitation MDCK 20413591
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Ƣ-catenin Co-immunoprecipitation COS-7 Mouse Full-length Not specified Not specified 20413591
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Ƣ-catenin Co-immunoprecipitation HEK 22374181
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Ƣ-tubulin-5 Co-immunofluorescence staining HEK293 Rat Full-length Rat Full-length 22309793
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Ƣ-tubulin-5 Co-immunoprecipitation HEK293 Rat Full-length Rat Full-length 22309793
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 HCK Co-immunoprecipitation HEK293 Mouse Full-length Not used 12538589
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 IP3R3 Co-immunoprecipitation HeLa Human Full-length Rat Full-length 18378772
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 IP3R3 Co-immunoprecipitation HEK293 Human Full-length Rat Full-length 18826956
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 ITCH/AIP4 Co-immunofluorescence staining HEK293T Mouse Full-length Mouse Full-length 17110928
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 ITCH/AIP4 Co-immunoprecipitation HEK293 Human Full-length Human Full-length 20650893
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 ITCH/AIP4 Co-immunoprecipitation HEK293T Mouse Full-length Mouse Full-length 17110928
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 LCK Co-immunoprecipitation HEK293 Mouse Full-length Not used 12538589
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 LYN Co-immunofluorescence staining HEK293 Mouse Full-length Not used 12538589
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 LYN Co-immunoprecipitation Rat dorsal root ganglion neuron 18234883
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 LYN Co-immunoprecipitation HEK293 Mouse Full-length Mouse Full-length 12538589
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 MAP7 Co-immunofluorescence staining Mouse lung section 14517216
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 MAP7 Co-immunofluorescence staining Mouse kidney section 14517216
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 MAP7 Co-immunofluorescence staining HEK293 Mouse Full-length Mouse Full-length 14517216
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 MAP7 Co-immunoprecipitation Mouse renal tissue 14517216
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 MAP7 Co-immunoprecipitation HEK293 Mouse 785-808 Mouse Full-length 14517216
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Myosin-10 Co-immunofluorescence staining HEK293 Mouse Full-length Not used 22492541
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Myosin-HIIA Co-immunofluorescence staining HEK293 Mouse Full-length Not used 22492541
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 ơ2-integrin Co-immunoprecipitation Rat dorsal root ganglion neuron 18234883
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 OS-9 Co-immunofluorescence staining SJSA-1 Mouse Full-length Not used 17932042
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 OS-9 Co-immunoprecipitation HEK293T Mouse 61-467 Human 430-667 17932042
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 PACS-1 Co-immunoprecipitation HEK293T Human Full-length Human Full-length 15692563
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Pacsin1 Co-immunoprecipitation HEK293 Rat Full-length Mouse Full-length 16627472
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Pacsin1 Co-immunoprecipitation HEK293 Mouse Full-length Mouse Full-length 16627472
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Pacsin2 Co-immunofluorescence staining HEK293 Rat Full-length Mouse Full-length 18174177
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Pacsin2 Co-immunoprecipitation HEK293 Rat Full-length Mouse Full-length 16627472
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Pacsin2 Co-immunoprecipitation HEK293 Mouse Full-length Mouse Full-length 16627472
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Pacsin3 Co-immunofluorescence staining Mouse kidney renal tubule 16627472
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Pacsin3 Co-immunofluorescence staining NIH-3T3 Rat Full-length Mouse Full-length 16627472
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Pacsin3 Co-immunofluorescence staining HEK293 Rat Full-length Mouse Full-length 16627472
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Pacsin3 Co-immunofluorescence staining HEK293 Rat Full-length Mouse Full-length 18174177
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Pacsin3 Co-immunoprecipitation Mouse kidney tissue 18174177
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Pacsin3 Co-immunoprecipitation HEK293 Rat Full-length Mouse Full-length 16627472
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Pacsin3 Co-immunoprecipitation HEK293 Mouse Full-length Mouse Full-length 16627472
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Pacsin3 Fluorescence resonance energy transfer HEK293 Mouse Full-length Mouse Full-length 23690576
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 SRC Co-immunoprecipitation HEK293 Mouse Full-length Not used 12538589
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 TMEM16A/ANO1 Co-immunoprecipitation HEK293T Mouse Full-length Mouse Full-length 24509911
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 TRPC1 Co-immunofluorescence staining HEK293 Mouse Full-length Human Full-length 20093626
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 TRPC1 Co-immunofluorescence staining Rat mesenteric artery endothelial cell lysates 20093626
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 TRPC1 Co-immunoprecipitation HEK293 Mouse Full-length Human Full-length 20093626
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 TRPC1 Co-immunoprecipitation Rat mesenteric artery endothelial cell lysates 20093626
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 TRPC1 Co-immunoprecipitation Rat mesenteric artery endothelial cell Mouse Full-length Human Full-length 25114176
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 TRPC1 Co-immunoprecipitation HEK293 Mouse Full-length Human Full-length 25114176
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 TRPC1 Co-immunoprecipitation Porcine coronary artery 25139746
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 TRPC1 Fluorescence resonance energy transfer HEK293 Mouse Full-length Human Full-length 20093626
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 TRPC1 Fluorescence resonance energy transfer HEK293 Mouse Full-length Human Full-length 20705915
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 TRPC1 Fluorescence resonance energy transfer HEK293 Mouse Full-length Human Full-length 21871867
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 TRPC1 Fluorescence resonance energy transfer HEK293 Mouse Full-length Human Full-length 25114176
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 TRPP1 Co-immunofluorescence staining MDCK 18695040
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 TRPP1 Co-immunofluorescence staining HEK293 Mouse Full-length Human Full-length 22518003
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 TRPP1 Co-immunofluorescence staining CRL-2038 (Mouse renal cortical collecting duct cell line / M1-CCD) Mouse Full-length Human Full-length 22518003
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 TRPP1 Co-immunofluorescence staining Mouse orpk collecting duct cell line 23977387
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 TRPP1 Co-immunoprecipitation HEK293 tsA 201 Mouse Full-length Human Full-length 20682256
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 TRPP1 Co-immunoprecipitation Rat mesenteric artery endothelial cell Mouse Full-length Human Full-length 25114176
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 TRPP1 Co-immunoprecipitation HEK293 Mouse Full-length Human Full-length 25114176
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 TRPP1 Co-immunoprecipitation HEK293 Mouse C-terminus Human C-terminus 18695040
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 TRPP1 Co-immunoprecipitation HEK293 Mouse Full-length Human Full-length 22518003
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 TRPP1 Co-immunoprecipitation CRL-2038 (Mouse renal cortical collecting duct cell line / M1-CCD) Mouse Full-length Human Full-length 22518003
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 TRPP1 Co-immunoprecipitation Mouse inner medullary collecting duct cell 23977387
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 TRPP1 Co-immunoprecipitation Mouse orpk collecting duct cell line 23977387
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 TRPP1 Fluorescence resonance energy transfer HEK293 Mouse Full-length Human Full-length 25114176
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 TRPP1 Fluorescence resonance energy transfer HEK293 Mouse Full-length Human Full-length 18695040
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Tubulin Co-immunoprecipitation HEK293 Mouse Full-length Not used 22101010
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 YES Co-immunoprecipitation HEK293 Mouse Full-length Not used 12538589
(Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Characterization Toggle 893349bafcc528f8346c51dc3420151d67b0126b2c122dd1017121c03fa0f69b
  Binding region mapping Stoichiometry Affinity (Kd) Reference
TRP channel Interactor
TRP channel Interactor Method Species Region Species Region
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Calmodulin Fusion protein-pull down assay Rat ARD domain Not specified Not determined 20926660
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Calmodulin Fusion protein-pull down assay Chicken 132-383 Not specified Not determined 19864432
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Calmodulin Fusion protein-pull down assay Human 718-871 Human Not determined 21837266
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Calmodulin Fusion protein-pull down assay Human 812-831 Not specified Not determined 12724311
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 FYN Co-immunoprecipitation Mouse Not determined Mouse 145-247 12538589
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 ƣ-actin Fusion protein-pull down assay Mouse 718-871 Human Not determined 22101010
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Ƣ-catenin Co-immunoprecipitation Mouse Not determined Not specified Not determined 20413591
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 IP3R3 Co-immunoprecipitation Human 812-831 Rat Not determined 18826956
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 OS-9 Co-immunoprecipitation Mouse 438-468 Human 430-667 17932042
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 PACS-1 Co-immunoprecipitation Human 1-486 Human Not determined 15692563
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Pacsin3 Co-immunoprecipitation Mouse 132-144 Mouse 368-424 16627472
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Pacsin3 Co-immunoprecipitation Rat 132-144 Mouse 368-424 16627472
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 TRPC1 Fluorescence resonance energy transfer 2:2 21871867
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 TRPP1 Atomic force microscopy(AFM) analysis 2:2 20682256
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Tubulin Fusion protein-pull down assay Mouse 718-871 Human Not determined 22101010
(Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Functional consequence Toggle 893349bafcc528f8346c51dc3420151d67b0126b2c122dd1017121c03fa0f69b
TRP channel Interactor Method Post-translational modification Subcellular trafficking Activity Reference
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 AKAP5/AKAP150 Patch clamp Activation 25005230
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Calmodulin Patch clamp Activation 12724311
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 FYN Calcium measurement Activation 12538589
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 FYN In vitro PTM assay Phosphorylation (Tyr-273) 12538589
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 HCK Calcium measurement Activation 12538589
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 HCK In vitro PTM assay Phosphorylation (Tyr-273) 12538589
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 IP3R3 Calcium measurement Activation 18378772
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 IP3R3 Calcium measurement Activation 18826956
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 IP3R3 Patch clamp Activation 18378772
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 IP3R3 Patch clamp Activation 18826956
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 ITCH/AIP4 Co-immunofluorescence staining Decrease in plasma membrane level 17110928
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 LCK Calcium measurement Activation 12538589
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 LCK In vitro PTM assay Phosphorylation (Tyr-273) 12538589
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 LYN Calcium measurement Activation 12538589
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 LYN In vitro PTM assay Phosphorylation (Tyr-273) 12538589
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 MAP7 Cell surface biotinylation Increase in plasma membrane level 14517216
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 MAP7 Patch clamp Activation 14517216
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 OS-9 Cell surface biotinylation Retain in the endoplasmic reticulum 17932042
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Pacsin3 Co-immunofluorescence staining Increase in plasma membrane level 16627472
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 Pacsin3 Patch clamp Inhibition 18174177
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 SRC Calcium measurement Activation 12538589
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 SRC Calcium measurement Activation 19033444
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 SRC In vitro PTM assay Phosphorylation (Tyr-273) 12538589
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 SRC In vivo PTM assay Phosphorylation (Tyr-110) 19033444
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 SRC In vivo PTM assay Phosphorylation (Tyr-805) 19033444
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 TRPC1 Calcium measurement Activation 20093626
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 TRPC1 Calcium measurement Activation 20705915
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 TRPC1 Cell surface biotinylation Increase in plasma membrane level 20705915
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 TRPC1 Co-immunofluorescence staining Increase in plasma membrane level 20705915
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 TRPC1 Patch clamp New channel creation 25114176
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 TRPP1 Calcium measurement New channel creation 22518003
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 TRPP1 Patch clamp New channel creation 25114176
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 TRPP1 Patch clamp New channel creation 18695040
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 UBC In vivo PTM assay Ubiquitination 17110928
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 UBC In vivo PTM assay Ubiquitination 17932042
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 YES Calcium measurement Activation 12538589
TRPV4 Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4 YES In vitro PTM assay Phosphorylation (Tyr-273) 12538589
(Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
TRP / Interactor

To prevent spam comments, we use reCAPTCHA. Please type correct words into the following box.