DIAP1/DRF1 / TRP channels
Name: Protein diaphanous homolog 1
(DIAP1/DRF1)
Official Symbol: DIAPH1
provided by HGNC
Function:
Acts in a Rho-dependent manner to recruit PFY1 to the membrane. Required for the assembly of F-actin structures, such as actin cables and stress fibers. Nucleates actin filaments. Binds to the barbed end of the actin filament and slows down actin polymerization and depolymerization. Required for cytokinesis, and transcriptional activation of the serum response factor. DFR proteins couple Rho and Src tyrosine kinase during signaling and the regulation of actin dynamics. Functions as a scaffold protein for MAPRE1 and APC to stabilize microtubules and promote cell migration (By similarity). Has neurite outgrowth promoting activity (By similarity). In hear cells, it may play a role in the regulation of actin polymerization in hair cells. The MEMO1-RHOA-DIAPH1 signaling pathway plays an important role in ERBB2-dependent stabilization of microtubules at the cell cortex. It controls the localization of APC and CLASP2 to the cell membrane, via the regulation of GSK3B activity. In turn, membrane-bound APC allows the localization of the MACF1 to the cell membrane, which is required for microtubule capture and stabilization. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the control of cell shape.
Source: Reorganizing the protein space at the Universal Protein Resource (UniProt)
Nucleic Acids Res. 40: D71-D75 (2012).
Species |
External DB
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Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM | |
Human
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1729![]() |
O60610![]() |
DIP-47456N![]() |
MINT-1198324![]() |
108073![]() |
O60610![]() |
hsa:1729![]() |
602121![]() |
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Mouse
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13367![]() |
O08808![]() |
DIP-29028N![]() |
MINT-4093098![]() |
199221![]() |
O08808![]() |
mmu:13367![]() |
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Rat | 307483![]() |
258727![]() |
rno:307483![]() |
PPI pairs:
Biological Process:
- GO:0030041
: actin filament polymerization
- GO:0071420
: cellular response to histamine
- GO:0007010
: cytoskeleton organization
- GO:0035372
: protein localization to microtubule
- GO:0008360
: regulation of cell shape
- GO:0032886
: regulation of microtubule-based process
- GO:0051279
: regulation of release of sequestered calcium ion into cytosol
- GO:0007605
: sensory perception of sound
Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
World Wide Web URL: http://www.geneontology.org/
Disease:
- OMIM:602121
: Deafness, autosomal dominant 1 (OMIM:124900
)
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine,
Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/
World Wide Web URL: http://omim.org/
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Screening
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Experimental screening | Non-experimental screening | Reference | ||||||||
TRP channel construct | Interactor source | |||||||||
TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | |||
TRPP1 |
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DIAP1/DRF1 | Yeast two-hybrid | Human | 851-968 | Mouse | Embryonic | cDNA library | 15123714 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Validation: In vivo validation
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Assay with endogenous proteins | Assay with overexpressed proteins | Reference | ||||||||
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | |||||||
TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
TRPP1 |
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DIAP1/DRF1 | Co-immunofluorescence staining | LLC-PK1 | 15123714 | |||||
TRPP1 |
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DIAP1/DRF1 | Co-immunoprecipitation | HEK293 | 15123714 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Characterization
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Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | |||||||
TRP channel | Interactor | |||||||||
TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
TRPP1 |
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DIAP1/DRF1 | Co-immunoprecipitation | Human | 679-968 | Mouse | 543-1203 | 15123714 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
