ơII-spectrin / TRP channels
Name:
ơII-spectrin
*
UniProtKB IDs are not shown, 1) if protein (or gene) isotypes are not specified in the referred articles, 2) if their IDs are not found in the UniprotKB database or by us
Species |
External DB
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Entrez Gene | UniprotKB | DIP | IntAct | MINT | BioGRID | STRING | IUPHAR-DB | KEGG | OMIM | |
Human | ||||||||||
Mouse | ||||||||||
Rat |
Biological Process:
No information in Gene Ontology
Source: The Gene Ontology Consortium. Gene ontology: tool for the unification of biology. Nat. Genet.. May 2000;25(1):25-9.
World Wide Web URL: http://www.geneontology.org/
World Wide Web URL: http://www.geneontology.org/
Disease:
No information in OMIM
Source: Online Mendelian Inheritance in Man, OMIM®. McKusick-Nathans Institute of Genetic Medicine,
Johns Hopkins University (Baltimore, MD), May, 2012.
World Wide Web URL: http://omim.org/
World Wide Web URL: http://omim.org/
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Screening
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Experimental screening | Non-experimental screening | Reference | ||||||||
TRP channel construct | Interactor source | |||||||||
TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | |||
TRPC4 |
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ơII-spectrin | Yeast two-hybrid | Human | 686-977 | Human | Brain | cDNA library | 18048348 | |
TRPC5 |
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ơII-spectrin | Affinity purification-mass spectrometry | Endogenous protein | Rat | Brain | Cerebral cortex lysates | 16025302 | ||
TRPV1 |
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ơII-spectrin | Affinity purification-mass spectrometry | Not used | Mouse | Dorsal root ganglion tissue | Lysates | 24036102 |
(
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Validation: In vitro validation
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Assay with recombinant proteins | Reference | |||||||||
TRP channel construct | Interactor construct | |||||||||
TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | ||
TRPC4 |
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ơII-spectrin | Fusion protein-pull down assay | Human | Full-length | HEK293 lysates | Human | 1998-2472 | E. coli | 18048348 |
(
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Validation: In vivo validation
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Assay with endogenous proteins | Assay with overexpressed proteins | Reference | ||||||||
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | |||||||
TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
TRPC4 |
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ơII-spectrin | Co-immunoprecipitation | HEK293 | Mouse | Full-length | Not used | 18048348 |
(
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Characterization
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Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | |||||||
TRP channel | Interactor | |||||||||
TRP channel | Interactor | Method | Species | Region | Species | Region | ||||
TRPC4 |
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ơII-spectrin | Fusion protein-pull down assay | Human | 730-758 | Human | 2202-2313 | 18048348 | ||
TRPC4 |
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ơII-spectrin | Yeast two-hybrid | Human | 686-859 | Human | 1998-2472 | 18048348 |
(
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Functional consequence
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TRP channel | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference | ||||||
TRPC4 |
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ơII-spectrin | Cell surface biotinylation | Increase in plasma membrane level | 18048348 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
