TRPV6 / Calmodulin
Interaction:
TRPV6
/
Calmodulin
/
Calmodulin
 Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
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Screening
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| Experimental screening | Non-experimental screening | Reference | |||||||
| TRP channel construct | Interactor source | ||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | ||
| TRPV6 | Calmodulin | Inference | Prediction | 11248124 | |||||
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Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
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| Validation: In vitro validation
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| Assay with recombinant proteins | Reference | ||||||||
| TRP channel construct | Interactor construct | ||||||||
| TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | |
| TRPV6 | Calmodulin | Fusion protein-pull down assay | Rabbit | Full-length | In vitro translation | Not specified | Full-length | Not specified | 15123711 |
| TRPV6 | Calmodulin | Fusion protein-pull down assay | Human | 584-725 | In vitro translation | Bovine | Full-length | E. coli | 11248124 |
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Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
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|
| Validation: In vivo validation
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| Assay with endogenous proteins | Assay with overexpressed proteins | Reference | |||||||
| Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | ||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | |||
| TRPV6 | Calmodulin | Co-immunoprecipitation | Xenopus oocyte | Rabbit | Full-length | Not specified | Full-length | 15123711 | |
| TRPV6 | Calmodulin | Fluorescence resonance energy transfer | HEK293 | Rat | Full-length | Not specified | Full-length | 16959851 | |
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Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
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| Characterization
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| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | ||||||
| TRP channel | Interactor | ||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | |||
| TRPV6 | Calmodulin | Fluorescence probe labeling | 1:1 | 43 nM | 12765696 | ||||
| TRPV6 | Calmodulin | Fluorescence probe labeling | 65 nM | 11248124 | |||||
| TRPV6 | Calmodulin | Fusion protein-pull down assay | Human | ARD domain | Not specified | Not determined | 20926660 | ||
| TRPV6 | Calmodulin | Fusion protein-pull down assay | Rabbit | 327-577 | Not specified | Not determined | 15123711 | ||
| TRPV6 | Calmodulin | Fusion protein-pull down assay | Rabbit | 88-97 | Not specified | Not determined | 15123711 | ||
| TRPV6 | Calmodulin | Fusion protein-pull down assay | Rabbit | 643-656 | Not specified | Not determined | 15123711 | ||
| TRPV6 | Calmodulin | Fusion protein-pull down assay | Human | 691-711 | Bovine | Not determined | 11248124 | ||
(:
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:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
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| Functional consequence
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| TRP channel | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference | |||||
| TRPV6 | Calmodulin | Calcium measurement | Inhibition | 16959851 | |||||||
| TRPV6 | Calmodulin | Patch clamp | Inhibition | 15123711 | |||||||
| TRPV6 | Calmodulin | Patch clamp | Inhibition | 11248124 | |||||||
(:
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)