TRPV5 / NHERF4
Interaction:
TRPV5
/
NHERF4
/
NHERF4
 Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
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Screening
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| Experimental screening | Non-experimental screening | Reference | |||||||
| TRP channel construct | Interactor source | ||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | ||
| TRPV5 | NHERF4 | Yeast two-hybrid | Mouse | C-terminus | Mouse | Kidney | cDNA library | 16565876 | |
(
:
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:
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|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
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|
| Validation: In vitro validation
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| Assay with recombinant proteins | Reference | ||||||||
| TRP channel construct | Interactor construct | ||||||||
| TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | |
| TRPV5 | NHERF4 | Fusion protein-pull down assay | Mouse | C-terminus | E. coli | Mouse | Full-length | In vitro translation | 16565876 |
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
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|
| Validation: In vivo validation
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|---|---|---|---|---|---|---|---|---|---|
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference | |||||||
| Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | ||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | |||
| TRPV5 | NHERF4 | Co-immunofluorescence staining | Xenopus oocyte | Mouse | Full-length | Mouse | Full-length | 16565876 | |
| TRPV5 | NHERF4 | Co-immunofluorescence staining | Mouse kidney section | 16565876 | |||||
| TRPV5 | NHERF4 | Co-immunoprecipitation | HEK293 | Mouse | Full-length | Mouse | Full-length | 16565876 | |
(:
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:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Characterization
|
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|---|---|---|---|---|---|---|---|---|---|
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | ||||||
| TRP channel | Interactor | ||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | |||
| TRPV5 | NHERF4 | Fusion protein-pull down assay | Mouse | 579-617 | Mouse | Not determined | 16565876 | ||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)