TRPV5 / Calmodulin
Interaction:
TRPV5
/
Calmodulin
/
Calmodulin
 Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
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Screening
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| Experimental screening | Non-experimental screening | Reference | |||||||
| TRP channel construct | Interactor source | ||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | ||
| TRPV5 | Calmodulin | Inference | Prediction | 15123711 | |||||
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Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
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| Validation: In vitro validation
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| Assay with recombinant proteins | Reference | ||||||||
| TRP channel construct | Interactor construct | ||||||||
| TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | |
| TRPV5 | Calmodulin | Fusion protein-pull down assay | Mouse | Full-length | In vitro translation | Not specified | Full-length | Not specified | 15123711 |
| TRPV5 | Calmodulin | Fusion protein-pull down assay | Mouse | 1-327 | In vitro translation | Not specified | Full-length | Not specified | 15123711 |
| TRPV5 | Calmodulin | Fusion protein-pull down assay | Mouse | 578-730 | In vitro translation | Not specified | Full-length | Not specified | 15123711 |
(:
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Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
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| Characterization
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| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | ||||||
| TRP channel | Interactor | ||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | |||
| TRPV5 | Calmodulin | Fluorescence probe labeling | Human | 587-616 | Not specified | Not determined | 306 nM | 20686800 | |
| TRPV5 | Calmodulin | Fusion protein-pull down assay | Human | ARD domain | Not specified | Not determined | 20926660 | ||
| TRPV5 | Calmodulin | Fusion protein-pull down assay | Human | 696-729 | Not specified | Not determined | 21576356 | ||
| TRPV5 | Calmodulin | Native-gel electrophoresis | Human | 696-729 | Not specified | Not determined | 21576356 | ||
(:
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|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
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| Functional consequence
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| TRP channel | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference | |||||
| TRPV5 | Calmodulin | Calcium measurement | Inhibition | 21576356 | |||||||
| TRPV5 | Calmodulin | Patch clamp | Inhibition | 21576356 | |||||||
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