TRPV4 / OS-9
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Screening
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Experimental screening | Non-experimental screening | Reference | |||||||
TRP channel construct | Interactor source | ||||||||
TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | ||
TRPV4 | OS-9 | Yeast two-hybrid | Mouse | 61-467 | Human | adult kidney | cDNA library | 17932042 |
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Validation: In vivo validation
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Assay with endogenous proteins | Assay with overexpressed proteins | Reference | |||||||
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | ||||||
TRP channel | Interactor | Method | Species | Region | Species | Region | |||
TRPV4 | OS-9 | Co-immunofluorescence staining | SJSA-1 | Mouse | Full-length | Not used | 17932042 | ||
TRPV4 | OS-9 | Co-immunoprecipitation | HEK293T | Mouse | 61-467 | Human | 430-667 | 17932042 |
(
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Characterization
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Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | ||||||
TRP channel | Interactor | ||||||||
TRP channel | Interactor | Method | Species | Region | Species | Region | |||
TRPV4 | OS-9 | Co-immunoprecipitation | Mouse | 438-468 | Human | 430-667 | 17932042 |
(
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Functional consequence
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TRP channel | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference | |||||
TRPV4 | OS-9 | Cell surface biotinylation | Retain in the endoplasmic reticulum | 17932042 |
(
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
