TRPV4 / OS-9
Interaction:
TRPV4
/
OS-9
/
OS-9
 Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
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Screening
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| Experimental screening | Non-experimental screening | Reference | |||||||
| TRP channel construct | Interactor source | ||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | ||
| TRPV4 | OS-9 | Yeast two-hybrid | Mouse | 61-467 | Human | adult kidney | cDNA library | 17932042 | |
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:
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Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
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|
| Validation: In vivo validation
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| Assay with endogenous proteins | Assay with overexpressed proteins | Reference | |||||||
| Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | ||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | |||
| TRPV4 | OS-9 | Co-immunofluorescence staining | SJSA-1 | Mouse | Full-length | Not used | 17932042 | ||
| TRPV4 | OS-9 | Co-immunoprecipitation | HEK293T | Mouse | 61-467 | Human | 430-667 | 17932042 | |
(:
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|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
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|
| Characterization
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| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | ||||||
| TRP channel | Interactor | ||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | |||
| TRPV4 | OS-9 | Co-immunoprecipitation | Mouse | 438-468 | Human | 430-667 | 17932042 | ||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Functional consequence
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| TRP channel | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference | |||||
| TRPV4 | OS-9 | Cell surface biotinylation | Retain in the endoplasmic reticulum | 17932042 | |||||||
(:
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