TRPV1 / Synaptotagmin 4
Interaction:
TRPV1
/
Synaptotagmin 4
/
Synaptotagmin 4
 Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
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Screening
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| Experimental screening | Non-experimental screening | Reference | |||||||
| TRP channel construct | Interactor source | ||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | ||
| TRPV1 | Synaptotagmin 4 | Yeast two-hybrid | Rat | 1-414 | Rat | Brain | cDNA library | 15066994 | |
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Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
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| Validation: In vitro validation
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| Assay with recombinant proteins | Reference | ||||||||
| TRP channel construct | Interactor construct | ||||||||
| TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | |
| TRPV1 | Synaptotagmin 4 | Fusion protein-pull down assay | Rat | 1-414 | E. coli | Human | Full-length | In vitro translation | 15066994 |
(:
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|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
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|
| Validation: In vivo validation
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| Assay with endogenous proteins | Assay with overexpressed proteins | Reference | |||||||
| Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | ||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | |||
| TRPV1 | Synaptotagmin 4 | Co-immunofluorescence staining | Rat dorsal root ganglion neuron | 15066994 | |||||
| TRPV1 | Synaptotagmin 4 | Co-immunoprecipitation | HEK293 | Human | Full-length | Human | Full-length | 15066994 | |
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Characterization
|
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|---|---|---|---|---|---|---|---|---|---|
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | ||||||
| TRP channel | Interactor | ||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | |||
| TRPV1 | Synaptotagmin 4 | Fusion protein-pull down assay | Rat | 1-414 | Human | C2A domain | 15066994 | ||
(:
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:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)