TRPV1 / FAF1
Interaction:
TRPV1
/
FAF1
/
FAF1
 Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
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Screening
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| Experimental screening | Non-experimental screening | Reference | |||||||
| TRP channel construct | Interactor source | ||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | ||
| TRPV1 | FAF1 | Yeast two-hybrid | Rat | 243-440 | Rat | Sensory neuron | cDNA expression library | 16510717 | |
(
:
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:
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Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
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| Validation: In vitro validation
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| Assay with recombinant proteins | Reference | ||||||||
| TRP channel construct | Interactor construct | ||||||||
| TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | |
| TRPV1 | FAF1 | Fusion protein-pull down assay | Rat | 1-440 | E. coli | Rat | Full-length | E. coli | 16510717 |
(:
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:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
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|
| Validation: In vivo validation
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| Assay with endogenous proteins | Assay with overexpressed proteins | Reference | |||||||
| Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | ||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | |||
| TRPV1 | FAF1 | Co-immunofluorescence staining | Rat dorsal root ganglion cell | 16510717 | |||||
| TRPV1 | FAF1 | Co-immunoprecipitation | Rat dorsal root ganglion cell | 16510717 | |||||
| TRPV1 | FAF1 | Co-immunoprecipitation | HEK293T | Rat | Full-length | Rat | Full-length | 16510717 | |
(:
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:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
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|
| Characterization
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|---|---|---|---|---|---|---|---|---|---|
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | ||||||
| TRP channel | Interactor | ||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | |||
| TRPV1 | FAF1 | Fusion protein-pull down assay | Rat | 243-440 | Rat | 110-169 | 16510717 | ||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
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|
| Functional consequence
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|---|---|---|---|---|---|---|---|---|---|---|---|
| TRP channel | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference | |||||
| TRPV1 | FAF1 | Calcium measurement | Inhibition | 16510717 | |||||||
| TRPV1 | FAF1 | Patch clamp | Inhibition | 16510717 | |||||||
(:
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:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)