TRPV1 / FAF1
![]() ![]() ![]() ![]() ![]() |
![]() |
Screening
![]() |
|||||||||
---|---|---|---|---|---|---|---|---|---|
Experimental screening | Non-experimental screening | Reference | |||||||
TRP channel construct | Interactor source | ||||||||
TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | ||
TRPV1 | FAF1 | Yeast two-hybrid | Rat | 243-440 | Rat | Sensory neuron | cDNA expression library | 16510717 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

![]() ![]() ![]() ![]() ![]() |
![]() |
Validation: In vitro validation
![]() |
|||||||||
---|---|---|---|---|---|---|---|---|---|
Assay with recombinant proteins | Reference | ||||||||
TRP channel construct | Interactor construct | ||||||||
TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | |
TRPV1 | FAF1 | Fusion protein-pull down assay | Rat | 1-440 | E. coli | Rat | Full-length | E. coli | 16510717 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

![]() ![]() ![]() ![]() ![]() |
![]() |
Validation: In vivo validation
![]() |
|||||||||
---|---|---|---|---|---|---|---|---|---|
Assay with endogenous proteins | Assay with overexpressed proteins | Reference | |||||||
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | ||||||
TRP channel | Interactor | Method | Species | Region | Species | Region | |||
TRPV1 | FAF1 | Co-immunofluorescence staining | Rat dorsal root ganglion cell | 16510717 | |||||
TRPV1 | FAF1 | Co-immunoprecipitation | Rat dorsal root ganglion cell | 16510717 | |||||
TRPV1 | FAF1 | Co-immunoprecipitation | HEK293T | Rat | Full-length | Rat | Full-length | 16510717 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

![]() ![]() ![]() ![]() ![]() |
![]() |
Characterization
![]() |
|||||||||
---|---|---|---|---|---|---|---|---|---|
Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | ||||||
TRP channel | Interactor | ||||||||
TRP channel | Interactor | Method | Species | Region | Species | Region | |||
TRPV1 | FAF1 | Fusion protein-pull down assay | Rat | 243-440 | Rat | 110-169 | 16510717 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

![]() ![]() ![]() ![]() ![]() |
![]() |
Functional consequence
![]() |
|||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
TRP channel | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference | |||||
TRPV1 | FAF1 | Calcium measurement | Inhibition | 16510717 | |||||||
TRPV1 | FAF1 | Patch clamp | Inhibition | 16510717 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
