TRPV1 / Eferin/Rab11-FIP3
Interaction:
TRPV1
/
Eferin/Rab11-FIP3
/
Eferin/Rab11-FIP3
 Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
Screening
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|
| Experimental screening | Non-experimental screening | Reference | |||||||
| TRP channel construct | Interactor source | ||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | ||
| TRPV1 | Eferin/Rab11-FIP3 | Yeast two-hybrid | Rat | 684-820 | Mouse | Brain | cDNA library | 15883036 | |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Validation: In vitro validation
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|
| Assay with recombinant proteins | Reference | ||||||||
| TRP channel construct | Interactor construct | ||||||||
| TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | |
| TRPV1 | Eferin/Rab11-FIP3 | Fusion protein-pull down assay | Rat | Full-length | HEK293T lysates | Mouse | Full-length | E. coli | 15883036 |
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Validation: In vivo validation
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference | |||||||
| Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | ||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | |||
| TRPV1 | Eferin/Rab11-FIP3 | Co-immunofluorescence staining | HEK293T | Rat | Full-length | Human | Full-length | 15883036 | |
| TRPV1 | Eferin/Rab11-FIP3 | Co-immunoprecipitation | HEK293T | Rat | Full-length | Human | Full-length | 15883036 | |
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Characterization
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | ||||||
| TRP channel | Interactor | ||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | |||
| TRPV1 | Eferin/Rab11-FIP3 | Yeast two-hybrid | Rat | 684-820 | Mouse | 345-444 | 15883036 | ||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)