TRPV1 / Calmodulin
Interaction:
TRPV1
/
Calmodulin
/
Calmodulin
 Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
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Screening
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| Experimental screening | Non-experimental screening | Reference | |||||||
| TRP channel construct | Interactor source | ||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | ||
| TRPV1 | Calmodulin | Inference | Prediction | 12808128 | |||||
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Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
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| Validation: In vitro validation
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| Assay with recombinant proteins | Reference | ||||||||
| TRP channel construct | Interactor construct | ||||||||
| TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | |
| TRPV1 | Calmodulin | Fast performance liquid chromatography | Rat | 759-802 | E. coli | Human | Full-length | E. coli | 23109716 |
| TRPV1 | Calmodulin | Fast performance liquid chromatography | Rat | 712?838 | E. coli | Mouse | Full-length | E. coli | 23119017 |
| TRPV1 | Calmodulin | Fusion protein-pull down assay | Rat | N-terminus | E. coli | Not specified | Full-length | In vitro translation | 14699077 |
| TRPV1 | Calmodulin | Fusion protein-pull down assay | Rat | C-terminus | E. coli | Bovine | Full-length | Not specified | 12808128 |
| TRPV1 | Calmodulin | X-ray diffraction-structural analysis | Rat | 784-798 | E. coli | Human | Full-length | E. coli | 23109716 |
(:
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|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
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|
| Validation: In vivo validation
|
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|---|---|---|---|---|---|---|---|---|---|
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference | |||||||
| Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | ||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | |||
| TRPV1 | Calmodulin | Co-immunoprecipitation | HEK293 | Rat | Full-length | Rat | Full-length | 12808128 | |
(:
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Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
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| Characterization
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|---|---|---|---|---|---|---|---|---|---|
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | ||||||
| TRP channel | Interactor | ||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | |||
| TRPV1 | Calmodulin | Fast performance liquid chromatography | Rat | 712?838 | Mouse | Not determined | 23119017 | ||
| TRPV1 | Calmodulin | Fusion protein-pull down assay | Rat | ARD domain | Not specified | Not determined | 20926660 | ||
| TRPV1 | Calmodulin | Fusion protein-pull down assay | Rat | 189-222 | Not specified | Not determined | 14699077 | ||
| TRPV1 | Calmodulin | Fusion protein-pull down assay | Human | 101-364 | Not specified | Not determined | 19864432 | ||
| TRPV1 | Calmodulin | Fusion protein-pull down assay | Rat | 767-801 | Bovine | Not determined | 12808128 | ||
| TRPV1 | Calmodulin | X-ray diffraction-structural analysis | Rat | 784-798 | Human | 11-145 | 23109716 | ||
(:
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:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
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|
| Functional consequence
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| TRP channel | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference | |||||
| TRPV1 | Calmodulin | Patch clamp | Inhibition | 14699077 | |||||||
| TRPV1 | Calmodulin | Patch clamp | Sensitization | 23109716 | |||||||
| TRPV1 | Calmodulin | Patch clamp | Inhibition | 12808128 | |||||||
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