TRPV1 / AKAP5/AKAP150

Interaction: TRPV1Detail bc8838fa5bd2464c5553a5a10de82d112f40db7d921ce377f414735e0504230c / AKAP5/AKAP150Detail bc8838fa5bd2464c5553a5a10de82d112f40db7d921ce377f414735e0504230c
Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b Screening Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b Functional consequence Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b top
Screening Toggle 893349bafcc528f8346c51dc3420151d67b0126b2c122dd1017121c03fa0f69b
  Experimental screening Non-experimental screening Reference
TRP channel construct Interactor source
TRP channel Interactor Method Species Region Species Organ/tissue Sample type
TRPV1 AKAP5/AKAP150 Inference Prediction 18381233
(Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b Screening Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b Functional consequence Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b top
Validation: In vitro validation Toggle 893349bafcc528f8346c51dc3420151d67b0126b2c122dd1017121c03fa0f69b
  Assay with recombinant proteins Reference
TRP channel construct Interactor construct
TRP channel Interactor Method Species Region Expression system Species Region Expression system
TRPV1 AKAP5/AKAP150 Fusion protein-pull down assay Human 681-839 E. coli Not used HEK293 lysates 18701070
(Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b Screening Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b Functional consequence Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b top
Validation: In vivo validation Toggle 893349bafcc528f8346c51dc3420151d67b0126b2c122dd1017121c03fa0f69b
  Assay with endogenous proteins Assay with overexpressed proteins Reference
Cell or tissue Cell or tissue TRP channel construct Interactor construct
TRP channel Interactor Method Species Region Species Region
TRPV1 AKAP5/AKAP150 Co-immunofluorescence staining Rat trigeminal ganglia neuron 18381233
TRPV1 AKAP5/AKAP150 Co-immunofluorescence staining Mouse dorsal root ganglion tissue 18463244
TRPV1 AKAP5/AKAP150 Co-immunofluorescence staining Rat dorsal root ganglion neuron 18701070
TRPV1 AKAP5/AKAP150 Co-immunoprecipitation Rat trigeminal ganglia neuron 22789851
TRPV1 AKAP5/AKAP150 Co-immunoprecipitation Rat trigeminal ganglia neuron 18381233
TRPV1 AKAP5/AKAP150 Co-immunoprecipitation CHO Rat Full-length Rat Full-length 18381233
TRPV1 AKAP5/AKAP150 Co-immunoprecipitation Mouse spinalcord tissue 18463244
TRPV1 AKAP5/AKAP150 Co-immunoprecipitation Mouse dorsal root ganglion tissue 18463244
TRPV1 AKAP5/AKAP150 Co-immunoprecipitation HEK293 Human Full-length Human Full-length 23264624
TRPV1 AKAP5/AKAP150 Co-immunoprecipitation HEK293 Human Full-length Mouse Full-length 23264624
TRPV1 AKAP5/AKAP150 Co-immunoprecipitation Rat dorsal root ganglion neuron 18701070
TRPV1 AKAP5/AKAP150 Co-immunoprecipitation HEK293 Human Full-length Not used 18701070
(Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b Screening Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b Functional consequence Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b top
Characterization Toggle 893349bafcc528f8346c51dc3420151d67b0126b2c122dd1017121c03fa0f69b
  Binding region mapping Stoichiometry Affinity (Kd) Reference
TRP channel Interactor
TRP channel Interactor Method Species Region Species Region
TRPV1 AKAP5/AKAP150 Co-immunoprecipitation Human Not determined Human 326-336 23699529
TRPV1 AKAP5/AKAP150 Co-immunoprecipitation Human 736-749 Human Not determined 18701070
TRPV1 AKAP5/AKAP150 Fluorescence resonance energy transfer Human Not determined Human 326-336 23699529
TRPV1 AKAP5/AKAP150 Fusion protein-pull down assay Human 736-749 Human Not determined 18701070
TRPV1 AKAP5/AKAP150 Patch clamp Human 738 ?745 Human Not determined 23616546
(Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b Screening Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b Validation: In vitro validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b Validation: In vivo validation Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b Characterization Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b Functional consequence Arrow2 1fb2b8a5ad55723bad55961567d092af30310a7cf44ded6f695fc2aa4753ec2b top
Functional consequence Toggle 893349bafcc528f8346c51dc3420151d67b0126b2c122dd1017121c03fa0f69b
TRP channel Interactor Method Post-translational modification Subcellular trafficking Activity Reference
TRPV1 AKAP5/AKAP150 Cell surface biotinylation Increase in plasma membrane level 18701070
TRPV1 AKAP5/AKAP150 Co-immunofluorescence staining Increase in plasma membrane level 23699529
TRPV1 AKAP5/AKAP150 Co-immunoprecipitation Increase in plasma membrane level 23616546
TRPV1 AKAP5/AKAP150 In vivo PTM assay Phosphorylation 18381233
TRPV1 AKAP5/AKAP150 Patch clamp Activation 18701070
(Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
TRP / Interactor

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