TRPP1 / PKD1/Polycystin-1

Interaction: TRPP1Detail bc8838fa5bd2464c5553a5a10de82d112f40db7d921ce377f414735e0504230c / PKD1/Polycystin-1Detail bc8838fa5bd2464c5553a5a10de82d112f40db7d921ce377f414735e0504230c
Screening Toggle 893349bafcc528f8346c51dc3420151d67b0126b2c122dd1017121c03fa0f69b
  Experimental screening Non-experimental screening Reference
TRP channel construct Interactor source
TRP channel Interactor Method Species Region Species Organ/tissue Sample type
TRPP1 PKD1/Polycystin-1 Affinity purification-mass spectrometry Mouse Not specified Mouse Vascular smooth muscle MOVAS lysates 19879844
TRPP1 PKD1/Polycystin-1 Inference Prediction 9171830
(Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Validation: In vitro validation Toggle 893349bafcc528f8346c51dc3420151d67b0126b2c122dd1017121c03fa0f69b
  Assay with recombinant proteins Reference
TRP channel construct Interactor construct
TRP channel Interactor Method Species Region Expression system Species Region Expression system
TRPP1 PKD1/Polycystin-1 Yeast two-hybrid Human 680-968 Yeast Human 1114-1339 Yeast 9192675
TRPP1 PKD1/Polycystin-1 Yeast two-hybrid Human 687-968 Yeast Human 4088-4302 Yeast 9171830
(Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Validation: In vivo validation Toggle 893349bafcc528f8346c51dc3420151d67b0126b2c122dd1017121c03fa0f69b
  Assay with endogenous proteins Assay with overexpressed proteins Reference
Cell or tissue Cell or tissue TRP channel construct Interactor construct
TRP channel Interactor Method Species Region Species Region
TRPP1 PKD1/Polycystin-1 Co-immunofluorescence staining CHO Human Full-length Human Full-length 15060061
TRPP1 PKD1/Polycystin-1 Co-immunofluorescence staining CHO Human Full-length Human Full-length 11140688
TRPP1 PKD1/Polycystin-1 Co-immunofluorescence staining PKD2-/- transgenic mouse kidney tissue Human Full-length Mouse Full-length 12840011
TRPP1 PKD1/Polycystin-1 Co-immunofluorescence staining Mouse vascular smooth muscle cell 12937304
TRPP1 PKD1/Polycystin-1 Co-immunoprecipitation COS-1 11901144
TRPP1 PKD1/Polycystin-1 Co-immunoprecipitation Human kidney lysates 11901144
TRPP1 PKD1/Polycystin-1 Co-immunoprecipitation HEK293T Human 680-968 Human 1114-1339 9192675
TRPP1 PKD1/Polycystin-1 Co-immunoprecipitation HEK293 Human Full-length Human Full-length 23390129
TRPP1 PKD1/Polycystin-1 Co-immunoprecipitation CHO Human Full-length Human Full-length 11140688
TRPP1 PKD1/Polycystin-1 Co-immunoprecipitation COS-7 Human Full-length Mouse 4182-4293 12840011
TRPP1 PKD1/Polycystin-1 Co-immunoprecipitation Mouse vascular smooth muscle cell 12937304
(Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Characterization Toggle 893349bafcc528f8346c51dc3420151d67b0126b2c122dd1017121c03fa0f69b
  Binding region mapping Stoichiometry Affinity (Kd) Reference
TRP channel Interactor
TRP channel Interactor Method Species Region Species Region
TRPP1 PKD1/Polycystin-1 Co-immunoprecipitation Human 680-968 Human 1227-1339 9192675
TRPP1 PKD1/Polycystin-1 Co-immunoprecipitation Human 742-968 Human 4227-4303 11140688
TRPP1 PKD1/Polycystin-1 Fusion protein-pull down assay Human 833-895 Human 4213-4248 19556541
TRPP1 PKD1/Polycystin-1 Fusion protein-pull down assay Human 821-927 Mouse 4183-4270 19726544
TRPP1 PKD1/Polycystin-1 Fusion protein-pull down assay Human 742-872 Human 4214-4248 9171830
TRPP1 PKD1/Polycystin-1 Fusion protein-pull down assay Human 681-968 Not specified 4077-4302 15692563
TRPP1 PKD1/Polycystin-1 Yeast two-hybrid Human 742-968 Human 1227-1339 9192675
TRPP1 PKD1/Polycystin-1 Yeast two-hybrid Human 822-895 Human 4124-4303 10097141
TRPP1 PKD1/Polycystin-1 Yeast two-hybrid Human 742-872 Human 4214-4248 9171830
(Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Functional consequence Toggle 893349bafcc528f8346c51dc3420151d67b0126b2c122dd1017121c03fa0f69b
TRP channel Interactor Method Post-translational modification Subcellular trafficking Activity Reference
TRPP1 PKD1/Polycystin-1 Co-immunofluorescence staining Increase in plasma membrane level 11140688
TRPP1 PKD1/Polycystin-1 Patch clamp New channel creation 11140688
(Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
TRP / Interactor

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