TRPP1 / KIF3B
Interaction:
TRPP1
/
KIF3B
/
KIF3B
 Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
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Screening
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| Experimental screening | Non-experimental screening | Reference | |||||||
| TRP channel construct | Interactor source | ||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | ||
| TRPP1 | KIF3B | Yeast two-hybrid | Human | 682-968 | Human | Heart | cDNA library | 16950792 | |
| TRPP1 | KIF3B | Yeast two-hybrid | Human | 682-968 | Human | Heart | cDNA library | 17008358 | |
(
:
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:
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|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
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|
| Validation: In vitro validation
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| Assay with recombinant proteins | Reference | ||||||||
| TRP channel construct | Interactor construct | ||||||||
| TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | |
| TRPP1 | KIF3B | Fusion protein-pull down assay | Human | 1-215 | E. coli | Not used | Mouse inner medullary collecting duct cell lysates | 17008358 | |
| TRPP1 | KIF3B | Fusion protein-pull down assay | Human | 682-968 | E. coli | Not used | Mouse inner medullary collecting duct cell lysates | 17008358 | |
(:
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:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Validation: In vivo validation
|
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| Assay with endogenous proteins | Assay with overexpressed proteins | Reference | |||||||
| Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | ||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | |||
| TRPP1 | KIF3B | Co-immunofluorescence staining | Mouse inner medullary collecting duct cell | 17008358 | |||||
| TRPP1 | KIF3B | Co-immunoprecipitation | MDCK | 17008358 | |||||
| TRPP1 | KIF3B | Co-immunoprecipitation | Mouse inner medullary collecting duct cell | 17008358 | |||||
| TRPP1 | KIF3B | Co-immunoprecipitation | Human kidney lysates | 17008358 | |||||
(:
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:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
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|
| Characterization
|
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|---|---|---|---|---|---|---|---|---|---|
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | ||||||
| TRP channel | Interactor | ||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | |||
| TRPP1 | KIF3B | Fusion protein-pull down assay | Human | 682-968 | Human | 407-747 | 17008358 | ||
| TRPP1 | KIF3B | Yeast two-hybrid | Human | 1-215 | Human | 407-747 | 17008358 | ||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)