TRPP1 / ơ-actinin-1
Interaction:
TRPP1
/
ơ-actinin-1
/
ơ-actinin-1
 Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
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Screening
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| Experimental screening | Non-experimental screening | Reference | |||||||
| TRP channel construct | Interactor source | ||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | ||
| TRPP1 | ơ-actinin-1 | Inference | Prediction | 15843396 | |||||
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|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
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|
| Validation: In vitro validation
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| Assay with recombinant proteins | Reference | ||||||||
| TRP channel construct | Interactor construct | ||||||||
| TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | |
| TRPP1 | ơ-actinin-1 | Fusion protein-pull down assay | Human | 1-215 | E. coli | Chicken | Not specified | Not specified | 15843396 |
| TRPP1 | ơ-actinin-1 | Fusion protein-pull down assay | Human | 821-968 | E. coli | Chicken | Not specified | Not specified | 15843396 |
(:
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
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|
| Validation: In vivo validation
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| Assay with endogenous proteins | Assay with overexpressed proteins | Reference | |||||||
| Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | ||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | |||
| TRPP1 | ơ-actinin-1 | Co-immunofluorescence staining | NIH-3T3 | 15843396 | |||||
| TRPP1 | ơ-actinin-1 | Co-immunofluorescence staining | MDCK | 15843396 | |||||
| TRPP1 | ơ-actinin-1 | Co-immunofluorescence staining | Mouse inner medullary collecting duct cell | 15843396 | |||||
| TRPP1 | ơ-actinin-1 | Co-immunoprecipitation | HEK293 | 15843396 | |||||
| TRPP1 | ơ-actinin-1 | Co-immunoprecipitation | MDCK | 15843396 | |||||
| TRPP1 | ơ-actinin-1 | Co-immunoprecipitation | Rat kidney tissue | 15843396 | |||||
(:
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:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Characterization
|
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|---|---|---|---|---|---|---|---|---|---|
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | ||||||
| TRP channel | Interactor | ||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | |||
| TRPP1 | ơ-actinin-1 | Fusion protein-pull down assay | Human | 821-878 | Chicken | Not determined | 15843396 | ||
| TRPP1 | ơ-actinin-1 | Yeast two-hybrid | Human | 821-878 | Human | 334-892 | 15843396 | ||
(:
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:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)