TRPP1 / ơ-actinin-1
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Screening
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Experimental screening | Non-experimental screening | Reference | |||||||
TRP channel construct | Interactor source | ||||||||
TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | ||
TRPP1 | ơ-actinin-1 | Inference | Prediction | 15843396 |
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Validation: In vitro validation
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Assay with recombinant proteins | Reference | ||||||||
TRP channel construct | Interactor construct | ||||||||
TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | |
TRPP1 | ơ-actinin-1 | Fusion protein-pull down assay | Human | 1-215 | E. coli | Chicken | Not specified | Not specified | 15843396 |
TRPP1 | ơ-actinin-1 | Fusion protein-pull down assay | Human | 821-968 | E. coli | Chicken | Not specified | Not specified | 15843396 |
(
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Validation: In vivo validation
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Assay with endogenous proteins | Assay with overexpressed proteins | Reference | |||||||
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | ||||||
TRP channel | Interactor | Method | Species | Region | Species | Region | |||
TRPP1 | ơ-actinin-1 | Co-immunofluorescence staining | NIH-3T3 | 15843396 | |||||
TRPP1 | ơ-actinin-1 | Co-immunofluorescence staining | MDCK | 15843396 | |||||
TRPP1 | ơ-actinin-1 | Co-immunofluorescence staining | Mouse inner medullary collecting duct cell | 15843396 | |||||
TRPP1 | ơ-actinin-1 | Co-immunoprecipitation | HEK293 | 15843396 | |||||
TRPP1 | ơ-actinin-1 | Co-immunoprecipitation | MDCK | 15843396 | |||||
TRPP1 | ơ-actinin-1 | Co-immunoprecipitation | Rat kidney tissue | 15843396 |
(
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Characterization
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Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | ||||||
TRP channel | Interactor | ||||||||
TRP channel | Interactor | Method | Species | Region | Species | Region | |||
TRPP1 | ơ-actinin-1 | Fusion protein-pull down assay | Human | 821-878 | Chicken | Not determined | 15843396 | ||
TRPP1 | ơ-actinin-1 | Yeast two-hybrid | Human | 821-878 | Human | 334-892 | 15843396 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
