TRPP1 / ơ-actinin-1

Interaction: TRPP1Detail bc8838fa5bd2464c5553a5a10de82d112f40db7d921ce377f414735e0504230c / ơ-actinin-1Detail bc8838fa5bd2464c5553a5a10de82d112f40db7d921ce377f414735e0504230c
Screening Toggle 893349bafcc528f8346c51dc3420151d67b0126b2c122dd1017121c03fa0f69b
  Experimental screening Non-experimental screening Reference
TRP channel construct Interactor source
TRP channel Interactor Method Species Region Species Organ/tissue Sample type
TRPP1 ơ-actinin-1 Inference Prediction 15843396
(Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Validation: In vitro validation Toggle 893349bafcc528f8346c51dc3420151d67b0126b2c122dd1017121c03fa0f69b
  Assay with recombinant proteins Reference
TRP channel construct Interactor construct
TRP channel Interactor Method Species Region Expression system Species Region Expression system
TRPP1 ơ-actinin-1 Fusion protein-pull down assay Human 1-215 E. coli Chicken Not specified Not specified 15843396
TRPP1 ơ-actinin-1 Fusion protein-pull down assay Human 821-968 E. coli Chicken Not specified Not specified 15843396
(Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Validation: In vivo validation Toggle 893349bafcc528f8346c51dc3420151d67b0126b2c122dd1017121c03fa0f69b
  Assay with endogenous proteins Assay with overexpressed proteins Reference
Cell or tissue Cell or tissue TRP channel construct Interactor construct
TRP channel Interactor Method Species Region Species Region
TRPP1 ơ-actinin-1 Co-immunofluorescence staining NIH-3T3 15843396
TRPP1 ơ-actinin-1 Co-immunofluorescence staining MDCK 15843396
TRPP1 ơ-actinin-1 Co-immunofluorescence staining Mouse inner medullary collecting duct cell 15843396
TRPP1 ơ-actinin-1 Co-immunoprecipitation HEK293 15843396
TRPP1 ơ-actinin-1 Co-immunoprecipitation MDCK 15843396
TRPP1 ơ-actinin-1 Co-immunoprecipitation Rat kidney tissue 15843396
(Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Characterization Toggle 893349bafcc528f8346c51dc3420151d67b0126b2c122dd1017121c03fa0f69b
  Binding region mapping Stoichiometry Affinity (Kd) Reference
TRP channel Interactor
TRP channel Interactor Method Species Region Species Region
TRPP1 ơ-actinin-1 Fusion protein-pull down assay Human 821-878 Chicken Not determined 15843396
TRPP1 ơ-actinin-1 Yeast two-hybrid Human 821-878 Human 334-892 15843396
(Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
TRP / Interactor

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