TRPM8 / Gơq
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Screening
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Experimental screening | Non-experimental screening | Reference | |||||||
TRP channel construct | Interactor source | ||||||||
TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | ||
TRPM8 | Gơq | Inference | Prediction | 22460725 |
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Validation: In vitro validation
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Assay with recombinant proteins | Reference | ||||||||
TRP channel construct | Interactor construct | ||||||||
TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | |
TRPM8 | Gơq | Fusion protein-pull down assay | Human | Full-length | HEK293 lysates | Human | Full-length | HEK293 lysates | 22750945 |
TRPM8 | Gơq | Fusion protein-pull down assay | Human | Full-length | E. coli | Human | Full-length | HEK293 lysates | 22750945 |
(
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Validation: In vivo validation
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Assay with endogenous proteins | Assay with overexpressed proteins | Reference | |||||||
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | ||||||
TRP channel | Interactor | Method | Species | Region | Species | Region | |||
TRPM8 | Gơq | Co-immunofluorescence staining | HEK293 | Not specified | Full-length | Not specified | Full-length | 22460725 | |
TRPM8 | Gơq | Co-immunoprecipitation | Mouse dorsal root ganglion cell | 22750945 | |||||
TRPM8 | Gơq | Co-immunoprecipitation | HEK293 | Rat | Full-length | Not used | 23508958 | ||
TRPM8 | Gơq | Fluorescence resonance energy transfer | HEK293 | Not specified | Full-length | Not specified | Full-length | 22460725 |
(
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Characterization
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Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | ||||||
TRP channel | Interactor | ||||||||
TRP channel | Interactor | Method | Species | Region | Species | Region | |||
TRPM8 | Gơq | Fusion protein-pull down assay | Human | 1-691 | Human | Not determined | 22750945 | ||
TRPM8 | Gơq | Fusion protein-pull down assay | Human | 980-1104 | Human | Not determined | 22750945 | ||
TRPM8 | Gơq | Fusion protein-pull down assay | Human | Not determined | Human | 222-245 | 22750945 |
(
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Functional consequence
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TRP channel | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference | |||||
TRPM8 | Gơq | Patch clamp | Inhibition | 22750945 |
(
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
