TRPM7 / PLCƣ1
Interaction:
TRPM7
/
PLCƣ1
/
PLCƣ1
 Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
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Screening
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| Experimental screening | Non-experimental screening | Reference | |||||||
| TRP channel construct | Interactor source | ||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | ||
| TRPM7 | PLCƣ1 | Inference | Prediction | 11941371 | |||||
| TRPM7 | PLCƢ1 | Yeast two-hybrid | Not used as a bait | Rat | Brain | cDNA library | 11161216 | ||
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:
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:
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|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
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|
| Validation: In vitro validation
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|---|---|---|---|---|---|---|---|---|---|
| Assay with recombinant proteins | Reference | ||||||||
| TRP channel construct | Interactor construct | ||||||||
| TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | |
| TRPM7 | PLCƢ1 | Fusion protein-pull down assay | Rat | 1352-1862 | E. coli | Rat | Full-length | HEK293T lysates | 11941371 |
| TRPM7 | PLCƢ1 | Fusion protein-pull down assay | Not specified | Kinase domain | E. coli | Not specified | Full-length | HEK293 lysates | 22180838 |
| TRPM7 | PLCƢ1 | Yeast two-hybrid | Rat | 1532-1862 | Yeast | Rat | 643-1216 | Yeast | 11941371 |
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
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|
| Characterization
|
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|---|---|---|---|---|---|---|---|---|---|
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | ||||||
| TRP channel | Interactor | ||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | |||
| TRPM7 | PLCƢ1 | Yeast two-hybrid | Rat | 1532-1862 | Rat | 643-797 | 11941371 | ||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Functional consequence
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|---|---|---|---|---|---|---|---|---|---|---|---|
| TRP channel | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference | |||||
| TRPM7 | PLCƢ1 | Patch clamp | Inhibition | 11941371 | |||||||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)