TRPM4 / 14-3-3ƣ
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Screening
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Experimental screening | Non-experimental screening | Reference | |||||||
TRP channel construct | Interactor source | ||||||||
TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | ||
TRPM4 | 14-3-3ƣ | Yeast two-hybrid | Human | 1-174 | Human | cDNA libray | 25047048 |
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Validation: In vivo validation
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Assay with endogenous proteins | Assay with overexpressed proteins | Reference | |||||||
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | ||||||
TRP channel | Interactor | Method | Species | Region | Species | Region | |||
TRPM4 | 14-3-3ƣ | Co-immunofluorescence staining | HEK293T | Human | Full-length | Human | Full-length | 25047048 | |
TRPM4 | 14-3-3ƣ | Co-immunoprecipitation | HEK293T | Human | 1-174 | Human | Full-length | 25047048 | |
TRPM4 | 14-3-3ƣ | Co-immunoprecipitation | HEK293T | Human | Full-length | Human | Full-length | 25047048 |
(
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Characterization
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Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | ||||||
TRP channel | Interactor | ||||||||
TRP channel | Interactor | Method | Species | Region | Species | Region | |||
TRPM4 | 14-3-3ƣ | Co-immunoprecipitation | Human | 1-174 | Human | Full-length | 25047048 |
(
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Functional consequence
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TRP channel | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference | |||||
TRPM4 | 14-3-3ƣ | Patch clamp | Activation | 25047048 |
(
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
