TRPC6 / RNF24
![]() ![]() ![]() ![]() ![]() |
![]() |
Screening
![]() |
|||||||||
---|---|---|---|---|---|---|---|---|---|
Experimental screening | Non-experimental screening | Reference | |||||||
TRP channel construct | Interactor source | ||||||||
TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | ||
TRPC6 | RNF24 | Yeast two-hybrid | Not specified | 3-259 | Human | Fetal brain | cDNA library | 17850865 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

![]() ![]() ![]() ![]() ![]() |
![]() |
Validation: In vitro validation
![]() |
|||||||||
---|---|---|---|---|---|---|---|---|---|
Assay with recombinant proteins | Reference | ||||||||
TRP channel construct | Interactor construct | ||||||||
TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | |
TRPC6 | RNF24 | Fusion protein-pull down assay | Not specified | 3-259 | In vitro translation | Human | 98-148 | E. coli | 17850865 |
TRPC6 | RNF24 | Yeast two-hybrid | Not specified | 3-259 | Yeast | Human | 98-148 | Yeast | 17850865 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

![]() ![]() ![]() ![]() ![]() |
![]() |
Validation: In vivo validation
![]() |
|||||||||
---|---|---|---|---|---|---|---|---|---|
Assay with endogenous proteins | Assay with overexpressed proteins | Reference | |||||||
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | ||||||
TRP channel | Interactor | Method | Species | Region | Species | Region | |||
TRPC6 | RNF24 | Co-immunofluorescence staining | HEK293 | Not specified | Full-length | Human | Full-length | 17850865 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

![]() ![]() ![]() ![]() ![]() |
![]() |
Characterization
![]() |
|||||||||
---|---|---|---|---|---|---|---|---|---|
Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | ||||||
TRP channel | Interactor | ||||||||
TRP channel | Interactor | Method | Species | Region | Species | Region | |||
TRPC6 | RNF24 | Yeast two-hybrid | Not specified | 3-130 | Human | 98-148 | 17850865 | ||
TRPC6 | RNF24 | Yeast two-hybrid | Not specified | 122-259 | Human | 98-148 | 17850865 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

![]() ![]() ![]() ![]() ![]() |
![]() |
Functional consequence
![]() |
|||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
TRP channel | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference | |||||
TRPC6 | RNF24 | Cell surface biotinylation | Decrease in plasma membrane level | 17850865 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
