TRPC6 / Calmodulin
![]() ![]() ![]() ![]() ![]() |
![]() |
Screening
![]() |
|||||||||
---|---|---|---|---|---|---|---|---|---|
Experimental screening | Non-experimental screening | Reference | |||||||
TRP channel construct | Interactor source | ||||||||
TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | ||
TRPC6 | Calmodulin | Inference | Prediction | 11290752 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

![]() ![]() ![]() ![]() ![]() |
![]() |
Validation: In vitro validation
![]() |
|||||||||
---|---|---|---|---|---|---|---|---|---|
Assay with recombinant proteins | Reference | ||||||||
TRP channel construct | Interactor construct | ||||||||
TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | |
TRPC6 | Calmodulin | Fusion protein-pull down assay | Mouse | 828-883 | E. coli | Not specified | Full-length | In vitro translation | 12379180 |
TRPC6 | Calmodulin | Fusion protein-pull down assay | Mouse | 1-396 | In vitro translation | Not specified | Full-length | E. coli | 12601176 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

![]() ![]() ![]() ![]() ![]() |
![]() |
Validation: In vivo validation
![]() |
|||||||||
---|---|---|---|---|---|---|---|---|---|
Assay with endogenous proteins | Assay with overexpressed proteins | Reference | |||||||
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | ||||||
TRP channel | Interactor | Method | Species | Region | Species | Region | |||
TRPC6 | Calmodulin | Co-immunoprecipitation | PC12D | 15994335 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

![]() ![]() ![]() ![]() ![]() |
![]() |
Characterization
![]() |
|||||||||
---|---|---|---|---|---|---|---|---|---|
Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | ||||||
TRP channel | Interactor | ||||||||
TRP channel | Interactor | Method | Species | Region | Species | Region | |||
TRPC6 | Calmodulin | Fluorescence probe labeling | 320 nM | 19932145 | |||||
TRPC6 | Calmodulin | Fluorescence probe labeling | Mouse | 843-872 | Not specified | Not determined | 290 nM | 11290752 | |
TRPC6 | Calmodulin | Fusion protein-pull down assay | Mouse | 838-872 | Not specified | Not determined | 11290752 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

![]() ![]() ![]() ![]() ![]() |
![]() |
Functional consequence
![]() |
|||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
TRP channel | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference | |||||
TRPC6 | Calmodulin | Calcium measurement | Activation | 12379180 | |||||||
TRPC6 | Calmodulin | Patch clamp | Activation | 12379180 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
