TRPC5 / NCS-1
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Screening
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Experimental screening | Non-experimental screening | Reference | |||||||
TRP channel construct | Interactor source | ||||||||
TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | ||
TRPC5 | NCS-1 | Inference | Prediction | 16469785 |
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Validation: In vitro validation
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Assay with recombinant proteins | Reference | ||||||||
TRP channel construct | Interactor construct | ||||||||
TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | |
TRPC5 | NCS-1 | Fusion protein-pull down assay | Human | 619-973 | E. coli | Human | Full-length | E. coli | 16469785 |
TRPC5 | NCS-1 | Yeast two-hybrid | Human | 619-973 | Yeast | Human | Full-length | Yeast | 16469785 |
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Validation: In vivo validation
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Assay with endogenous proteins | Assay with overexpressed proteins | Reference | |||||||
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | ||||||
TRP channel | Interactor | Method | Species | Region | Species | Region | |||
TRPC5 | NCS-1 | Co-immunoprecipitation | HEK293 | Human | Full-length | Human | Full-length | 16469785 | |
TRPC5 | NCS-1 | Co-immunoprecipitation | Rat brain membrane lysates | 16469785 |
(
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Characterization
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Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | ||||||
TRP channel | Interactor | ||||||||
TRP channel | Interactor | Method | Species | Region | Species | Region | |||
TRPC5 | NCS-1 | Fusion protein-pull down assay | Human | 619-803 | Human | Not determined | 16469785 | ||
TRPC5 | NCS-1 | Fusion protein-pull down assay | Human | 803-973 | Human | Not determined | 16469785 | ||
TRPC5 | NCS-1 | Yeast two-hybrid | Human | 619-803 | Human | Not determined | 16469785 | ||
TRPC5 | NCS-1 | Yeast two-hybrid | Human | 803-973 | Human | Not determined | 16469785 |
(
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Functional consequence
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TRP channel | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference | |||||
TRPC5 | NCS-1 | Calcium measurement | Activation | 16469785 | |||||||
TRPC5 | NCS-1 | Patch clamp | Activation | 16469785 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
