TRPC5 / Calmodulin
Interaction:
TRPC5
/
Calmodulin
/
Calmodulin
 Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
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Screening
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| Experimental screening | Non-experimental screening | Reference | |||||||
| TRP channel construct | Interactor source | ||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | ||
| TRPC5 | Calmodulin | Inference | Prediction | 11290752 | |||||
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Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
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| Validation: In vitro validation
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| Assay with recombinant proteins | Reference | ||||||||
| TRP channel construct | Interactor construct | ||||||||
| TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | |
| TRPC5 | Calmodulin | Fusion protein-pull down assay | Mouse | 1-331 | In vitro translation | Not specified | Not specified | Not specified | 15987684 |
| TRPC5 | Calmodulin | Fusion protein-pull down assay | Mouse | 701-807 | In vitro translation | Not specified | Not specified | Not specified | 15987684 |
| TRPC5 | Calmodulin | Fusion protein-pull down assay | Mouse | 762-975 | In vitro translation | Not specified | Not specified | Not specified | 15987684 |
| TRPC5 | Calmodulin | Fusion protein-pull down assay | Mouse | 1-321 | In vitro translation | Not specified | Full-length | E. coli | 12601176 |
(:
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
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| Characterization
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| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | ||||||
| TRP channel | Interactor | ||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | |||
| TRPC5 | Calmodulin | Fluorescence probe labeling | Mouse | 702-731 | Not specified | Not determined | 220 nM | 11290752 | |
| TRPC5 | Calmodulin | Fusion protein-pull down assay | Mouse | 701-733 | Bovine | Not determined | 15987684 | ||
| TRPC5 | Calmodulin | Fusion protein-pull down assay | Mouse | 828-854 | Bovine | Not determined | 15987684 | ||
| TRPC5 | Calmodulin | Fusion protein-pull down assay | Mouse | 701-733 | Not specified | Not determined | 11290752 | ||
(:
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:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)