TRPC4 / NHERF1

Interaction: TRPC4Detail bc8838fa5bd2464c5553a5a10de82d112f40db7d921ce377f414735e0504230c / NHERF1Detail bc8838fa5bd2464c5553a5a10de82d112f40db7d921ce377f414735e0504230c
Screening Toggle 893349bafcc528f8346c51dc3420151d67b0126b2c122dd1017121c03fa0f69b
  Experimental screening Non-experimental screening Reference
TRP channel construct Interactor source
TRP channel Interactor Method Species Region Species Organ/tissue Sample type
TRPC4 NHERF1 Inference Prediction 10980202
TRPC4 NHERF1 Yeast two-hybrid Human 686-977 Human Brain cDNA library 18048348
(Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Validation: In vitro validation Toggle 893349bafcc528f8346c51dc3420151d67b0126b2c122dd1017121c03fa0f69b
  Assay with recombinant proteins Reference
TRP channel construct Interactor construct
TRP channel Interactor Method Species Region Expression system Species Region Expression system
TRPC4 NHERF1 Fusion protein-pull down assay Human Full-length HEK293 lysates Human 1-102 E. coli 12154080
TRPC4 NHERF1 Fusion protein-pull down assay Human Full-length HEK293 lysates Human 144-247 E. coli 12154080
TRPC4 NHERF1 Fusion protein-pull down assay Mouse Full-length In vitro translation Mouse 1-107 E. coli 10980202
(Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Validation: In vivo validation Toggle 893349bafcc528f8346c51dc3420151d67b0126b2c122dd1017121c03fa0f69b
  Assay with endogenous proteins Assay with overexpressed proteins Reference
Cell or tissue Cell or tissue TRP channel construct Interactor construct
TRP channel Interactor Method Species Region Species Region
TRPC4 NHERF1 Co-immunofluorescence staining COS-7 Human Full-length Not specifed Full-length 16144838
TRPC4 NHERF1 Co-immunoprecipitation HEK293 Mouse Full-length Not used 18048348
TRPC4 NHERF1 Co-immunoprecipitation HEK293 Human Full-length Human Full-length 12154080
TRPC4 NHERF1 Co-immunoprecipitation COS-7 Human Full-length Not specifed Full-length 16144838
TRPC4 NHERF1 Co-immunoprecipitation Human fetal brain astrocytes 15540229
TRPC4 NHERF1 Co-immunoprecipitation HEK293 Mouse Full-length Not used 10980202
TRPC4 NHERF1 Co-immunoprecipitation Mouse brain tissue 10980202
(Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Characterization Toggle 893349bafcc528f8346c51dc3420151d67b0126b2c122dd1017121c03fa0f69b
  Binding region mapping Stoichiometry Affinity (Kd) Reference
TRP channel Interactor
TRP channel Interactor Method Species Region Species Region
TRPC4 NHERF1 Co-immunoprecipitation Human 975-977 (in ơ varint) Human 130-328 12154080
TRPC4 NHERF1 Fusion protein-pull down assay Human 975-977 (in ơ varint) Human 1-102 12154080
TRPC4 NHERF1 Fusion protein-pull down assay Human 975-977 (in ơ varint) Human 144-247 12154080
TRPC4 NHERF1 Fusion protein-pull down assay Mouse 688-974 Mouse 1-107 10980202
(Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
Functional consequence Toggle 893349bafcc528f8346c51dc3420151d67b0126b2c122dd1017121c03fa0f69b
TRP channel Interactor Method Post-translational modification Subcellular trafficking Activity Reference
TRPC4 NHERF1 Cell surface biotinylation Increase in plasma membrane level 12154080
(Link 2bd4d11adb659cddf58197a94e201f0a44c55d8d7cb427c624971b42e122c0a4: click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
TRP / Interactor

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