TRPC4 / IP3R3
Interaction:
TRPC4
/
IP3R3
/
IP3R3
 Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
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Screening
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| Experimental screening | Non-experimental screening | Reference | |||||||
| TRP channel construct | Interactor source | ||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | ||
| TRPC4 | IP3R3 | Inference | Prediction | 11290752 | |||||
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|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
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|
| Validation: In vitro validation
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| Assay with recombinant proteins | Reference | ||||||||
| TRP channel construct | Interactor construct | ||||||||
| TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | |
| TRPC4 | IP3R3 | Fusion protein-pull down assay | Mouse | 688-974 | In vitro translation | Human | 669-698 | E. coli | 11290752 |
| TRPC4 | IP3R3 | Yeast two-hybrid | Human | 615-977 | Yeast | Rat | 2556-2713 | Yeast | 11163362 |
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Validation: In vivo validation
|
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| Assay with endogenous proteins | Assay with overexpressed proteins | Reference | |||||||
| Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | ||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | |||
| TRPC4 | IP3R3 | Co-immunoprecipitation | HEK293 | Mouse | Full-length | Not used | 14505576 | ||
(:
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:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Characterization
|
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|---|---|---|---|---|---|---|---|---|---|
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | ||||||
| TRP channel | Interactor | ||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | |||
| TRPC4 | IP3R3 | Fusion protein-pull down assay | Mouse | 695-724 | Human | 669-698 | 15104175 | ||
| TRPC4 | IP3R3 | Fusion protein-pull down assay | Mouse | 781-864 | Human | 669-698 | 11290752 | ||
| TRPC4 | IP3R3 | Fusion protein-pull down assay | Mouse | 695-724 | Human | 669-698 | 11290752 | ||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)