TRPC4 / Caveolin-1
Interaction:
TRPC4
/
Caveolin-1
/
Caveolin-1
 Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
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Screening
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| Experimental screening | Non-experimental screening | Reference | |||||||
| TRP channel construct | Interactor source | ||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | ||
| TRPC4 | Caveolin-1 | Inference | Prediction | 17416589 | |||||
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Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
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|
| Validation: In vitro validation
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| Assay with recombinant proteins | Reference | ||||||||
| TRP channel construct | Interactor construct | ||||||||
| TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | |
| TRPC4 | Caveolin-1 | Fusion protein-pull down assay | Not used | Mouse lung endothelium cell lysates | Not specified | Full-length | E. coli | 17416589 | |
(:
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|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
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|
| Validation: In vivo validation
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| Assay with endogenous proteins | Assay with overexpressed proteins | Reference | |||||||
| Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | ||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | |||
| TRPC4 | Caveolin-1 | Co-immunofluorescence staining | Mouse lung endothelium cell | 17416589 | |||||
| TRPC4 | Caveolin-1 | Co-immunoprecipitation | Mouse lung endothelium cell | 17416589 | |||||
(:
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:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Characterization
|
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|---|---|---|---|---|---|---|---|---|---|
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | ||||||
| TRP channel | Interactor | ||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | |||
| TRPC4 | Caveolin-1 | Fusion protein-pull down assay | Mouse | Not determined | Not specified | 61-101 | 17416589 | ||
| TRPC4 | Caveolin-1 | Fusion protein-pull down assay | Mouse | Not determined | Not specified | 135-178 | 17416589 | ||
(:
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:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)