TRPC4 / ơII-spectrin
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Screening
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Experimental screening | Non-experimental screening | Reference | |||||||
TRP channel construct | Interactor source | ||||||||
TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | ||
TRPC4 | ơII-spectrin | Yeast two-hybrid | Human | 686-977 | Human | Brain | cDNA library | 18048348 |
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Validation: In vitro validation
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Assay with recombinant proteins | Reference | ||||||||
TRP channel construct | Interactor construct | ||||||||
TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | |
TRPC4 | ơII-spectrin | Fusion protein-pull down assay | Human | Full-length | HEK293 lysates | Human | 1998-2472 | E. coli | 18048348 |
(
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Validation: In vivo validation
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Assay with endogenous proteins | Assay with overexpressed proteins | Reference | |||||||
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | ||||||
TRP channel | Interactor | Method | Species | Region | Species | Region | |||
TRPC4 | ơII-spectrin | Co-immunoprecipitation | HEK293 | Mouse | Full-length | Not used | 18048348 |
(
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Characterization
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Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | ||||||
TRP channel | Interactor | ||||||||
TRP channel | Interactor | Method | Species | Region | Species | Region | |||
TRPC4 | ơII-spectrin | Fusion protein-pull down assay | Human | 730-758 | Human | 2202-2313 | 18048348 | ||
TRPC4 | ơII-spectrin | Yeast two-hybrid | Human | 686-859 | Human | 1998-2472 | 18048348 |
(
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Functional consequence
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TRP channel | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference | |||||
TRPC4 | ơII-spectrin | Cell surface biotinylation | Increase in plasma membrane level | 18048348 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
