TRPC3 / VAMP2
Interaction:
TRPC3
/
VAMP2
/
VAMP2
 Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
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Screening
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| Experimental screening | Non-experimental screening | Reference | |||||||
| TRP channel construct | Interactor source | ||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | ||
| TRPC3 | VAMP2 | Affinity purification-mass spectrometry | Not used as a bait | Rat | Brain | Crude membranes | 18205297 | ||
| TRPC3 | VAMP2 | Yeast two-hybrid | Human | 1-341 | Rat | Brain | cDNA library | 15327778 | |
(
:
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:
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|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
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|
| Validation: In vivo validation
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| Assay with endogenous proteins | Assay with overexpressed proteins | Reference | |||||||
| Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | ||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | |||
| TRPC3 | VAMP2 | Co-immunofluorescence staining | Rat hippocampal neuron | 15327778 | |||||
| TRPC3 | VAMP2 | Co-immunofluorescence staining | HSG cell | 15327778 | |||||
| TRPC3 | VAMP2 | Co-immunofluorescence staining | HEK293 | Human | Full-length | Not used | 15327778 | ||
| TRPC3 | VAMP2 | Co-immunoprecipitation | Rat hippocampal neuron | 15327778 | |||||
| TRPC3 | VAMP2 | Co-immunoprecipitation | HEK293 | Human | Full-length | Not used | 15327778 | ||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Characterization
|
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|---|---|---|---|---|---|---|---|---|---|
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | ||||||
| TRP channel | Interactor | ||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | |||
| TRPC3 | VAMP2 | Yeast two-hybrid | Human | 1-123 | Rat | Not determined | 15327778 | ||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)