TRPC3 / RACK1
Interaction:
TRPC3
/
RACK1
/
RACK1
 Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
Screening
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|
| Experimental screening | Non-experimental screening | Reference | |||||||
| TRP channel construct | Interactor source | ||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | ||
| TRPC3 | RACK1 | Yeast two-hybrid | Not specified | 1-341 | Human | Embroynic kidney | cDNA library | 18755685 | |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Validation: In vivo validation
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|
| Assay with endogenous proteins | Assay with overexpressed proteins | Reference | |||||||
| Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | ||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | |||
| TRPC3 | RACK1 | Co-immunofluorescence staining | HEK293 | Not specified | Full-length | Not used | 18755685 | ||
| TRPC3 | RACK1 | Co-immunoprecipitation | HEK293 | Not specified | Full-length | Not used | 18755685 | ||
| TRPC3 | RACK1 | Co-immunoprecipitation | Rat brain lysates | 18755685 | |||||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Characterization
|
|||||||||
|---|---|---|---|---|---|---|---|---|---|
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | ||||||
| TRP channel | Interactor | ||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | |||
| TRPC3 | RACK1 | Yeast two-hybrid | Not specified | 221-342 | Human | Not determined | 18755685 | ||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)