TRPC3 / RACK1
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Screening
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Experimental screening | Non-experimental screening | Reference | |||||||
TRP channel construct | Interactor source | ||||||||
TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | ||
TRPC3 | RACK1 | Yeast two-hybrid | Not specified | 1-341 | Human | Embroynic kidney | cDNA library | 18755685 |
(
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Validation: In vivo validation
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Assay with endogenous proteins | Assay with overexpressed proteins | Reference | |||||||
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | ||||||
TRP channel | Interactor | Method | Species | Region | Species | Region | |||
TRPC3 | RACK1 | Co-immunofluorescence staining | HEK293 | Not specified | Full-length | Not used | 18755685 | ||
TRPC3 | RACK1 | Co-immunoprecipitation | HEK293 | Not specified | Full-length | Not used | 18755685 | ||
TRPC3 | RACK1 | Co-immunoprecipitation | Rat brain lysates | 18755685 |
(
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Characterization
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Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | ||||||
TRP channel | Interactor | ||||||||
TRP channel | Interactor | Method | Species | Region | Species | Region | |||
TRPC3 | RACK1 | Yeast two-hybrid | Not specified | 221-342 | Human | Not determined | 18755685 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
