TRPC3 / Orai1
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Screening
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Experimental screening | Non-experimental screening | Reference | |||||||
TRP channel construct | Interactor source | ||||||||
TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | ||
TRPC3 | Orai1 | Inference | Prediction | 17360584 |
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Validation: In vitro validation
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Assay with recombinant proteins | Reference | ||||||||
TRP channel construct | Interactor construct | ||||||||
TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | |
TRPC3 | Orai1 | Fusion protein-pull down assay | Human | 1-340 | In vitro translation | Human | Full-length | E. coli | 17360584 |
TRPC3 | Orai1 | Fusion protein-pull down assay | Human | 676-848 | In vitro translation | Human | Full-length | E. coli | 17360584 |
(
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Validation: In vivo validation
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Assay with endogenous proteins | Assay with overexpressed proteins | Reference | |||||||
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | ||||||
TRP channel | Interactor | Method | Species | Region | Species | Region | |||
TRPC3 | Orai1 | Co-immunoprecipitation | COS-7 | Human | Full-length | Human | Full-length | 17360584 | |
TRPC3 | Orai1 | Co-immunoprecipitation | HEK293 | 20022948 |
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Functional consequence
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TRP channel | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference | |||||
TRPC3 | Orai1 | Patch clamp | Activation | 18287061 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
