TRPC3 / NCX1
Interaction:
TRPC3
/
NCX1
/
NCX1
 Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
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Screening
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| Experimental screening | Non-experimental screening | Reference | |||||||
| TRP channel construct | Interactor source | ||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | ||
| TRPC3 | NCX1 | Inference | Prediction | 14736881 | |||||
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Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
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| Validation: In vitro validation
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| Assay with recombinant proteins | Reference | ||||||||
| TRP channel construct | Interactor construct | ||||||||
| TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | |
| TRPC3 | NCX1 | Fusion protein-pull down assay | Human | 742-848 | E. coli | Not used | HEK293 lysates | 14736881 | |
| TRPC3 | NCX1 | Fusion protein-pull down assay | Human | 670-848 | E. coli | Not used | Rat Cardiomyocyte lysates | 17129578 | |
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
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|
| Validation: In vivo validation
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| Assay with endogenous proteins | Assay with overexpressed proteins | Reference | |||||||
| Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | ||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | |||
| TRPC3 | NCX1 | Co-immunofluorescence staining | Rat myocyte section | 17012351 | |||||
| TRPC3 | NCX1 | Co-immunofluorescence staining | Rat cardiomyocytes | 17129578 | |||||
| TRPC3 | NCX1 | Co-immunofluorescence staining | dnTRPC3 TG mouse heart myocyte | 20351294 | |||||
| TRPC3 | NCX1 | Co-immunoprecipitation | HEK293 | Human | Full-length | Not used | 14736881 | ||
| TRPC3 | NCX1 | Co-immunoprecipitation | Sf9 | Mouse | Not specified | Rat | Not specified | 17012351 | |
| TRPC3 | NCX1 | Co-immunoprecipitation | Rat ventricle lysates | 17012351 | |||||
| TRPC3 | NCX1 | Co-immunoprecipitation | Rat cardiomyocytes | 17129578 | |||||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Characterization
|
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|---|---|---|---|---|---|---|---|---|---|
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | ||||||
| TRP channel | Interactor | ||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | |||
| TRPC3 | NCX1 | Fusion protein-pull down assay | Human | 670-848 | Rat | Not determined | 17129578 | ||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)