TRPC3 / Junctophilin-2
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Screening
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Experimental screening | Non-experimental screening | Reference | |||||||
TRP channel construct | Interactor source | ||||||||
TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | ||
TRPC3 | Junctophilin-2 | Inference | Prediction | 18215135 |
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Validation: In vitro validation
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Assay with recombinant proteins | Reference | ||||||||
TRP channel construct | Interactor construct | ||||||||
TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | |
TRPC3 | Junctophilin-2 | Fusion protein-pull down assay | Not used | Rabbit back and leg fast-twich skeletal muscle lysates | Mouse | Full-length | E. coli | 19277847 |
(
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Validation: In vivo validation
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Assay with endogenous proteins | Assay with overexpressed proteins | Reference | |||||||
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | ||||||
TRP channel | Interactor | Method | Species | Region | Species | Region | |||
TRPC3 | Junctophilin-2 | Co-immunoprecipitation | Mouse skeletal muscle myoblast lysates | Not used | Mouse | Full-length | 20095964 | ||
TRPC3 | Junctophilin-2 | Co-immunoprecipitation | Mouse skeletal myotube lysates | 18215135 |
(
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Characterization
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Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | ||||||
TRP channel | Interactor | ||||||||
TRP channel | Interactor | Method | Species | Region | Species | Region | |||
TRPC3 | Junctophilin-2 | Fusion protein-pull down assay | Mouse | Not determined | Mouse | 143-234 | 19277847 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
