TRPC3 / Junctophilin-2
Interaction:
TRPC3
/
Junctophilin-2
/
Junctophilin-2
 Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
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Screening
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| Experimental screening | Non-experimental screening | Reference | |||||||
| TRP channel construct | Interactor source | ||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | ||
| TRPC3 | Junctophilin-2 | Inference | Prediction | 18215135 | |||||
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Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
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|
| Validation: In vitro validation
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| Assay with recombinant proteins | Reference | ||||||||
| TRP channel construct | Interactor construct | ||||||||
| TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | |
| TRPC3 | Junctophilin-2 | Fusion protein-pull down assay | Not used | Rabbit back and leg fast-twich skeletal muscle lysates | Mouse | Full-length | E. coli | 19277847 | |
(:
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|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
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|
| Validation: In vivo validation
|
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| Assay with endogenous proteins | Assay with overexpressed proteins | Reference | |||||||
| Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | ||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | |||
| TRPC3 | Junctophilin-2 | Co-immunoprecipitation | Mouse skeletal muscle myoblast lysates | Not used | Mouse | Full-length | 20095964 | ||
| TRPC3 | Junctophilin-2 | Co-immunoprecipitation | Mouse skeletal myotube lysates | 18215135 | |||||
(:
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|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
top
|
| Characterization
|
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|---|---|---|---|---|---|---|---|---|---|
| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | ||||||
| TRP channel | Interactor | ||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | |||
| TRPC3 | Junctophilin-2 | Fusion protein-pull down assay | Mouse | Not determined | Mouse | 143-234 | 19277847 | ||
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