TRPC3 / IP3R1
Interaction:
TRPC3
/
IP3R1
/
IP3R1
 Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
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Screening
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| Experimental screening | Non-experimental screening | Reference | |||||||
| TRP channel construct | Interactor source | ||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | ||
| TRPC3 | IP3R1 | Affinity purification-mass spectrometry | Not used as a bait | Rat | Brain | Crude membranes | 18205297 | ||
| TRPC3 | IP3R1 | Inference | Prediction | 10518223 | |||||
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Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
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| Validation: In vivo validation
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| Assay with endogenous proteins | Assay with overexpressed proteins | Reference | |||||||
| Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | ||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | |||
| TRPC3 | IP3R1 | Co-immunoprecipitation | HEK293 | Human | Full-length | Not used | 11524429 | ||
| TRPC3 | IP3R1 | Co-immunoprecipitation | Rat cerebral arterial lysates | 20378853 | |||||
| TRPC3 | IP3R1 | Co-immunoprecipitation | Rat mesenteric artery lysates | 23045459 | |||||
| TRPC3 | IP3R1 | Co-immunoprecipitation | Rat brain lysates | 18205297 | |||||
| TRPC3 | IP3R1 | Co-immunoprecipitation | HEK293 | Human | Full-length | Rat | Full-length | 10518223 | |
| TRPC3 | IP3R1 | Co-immunoprecipitation | MDCK | Not specified | Full-length | Not used | 15623527 | ||
| TRPC3 | IP3R1 | Co-immunoprecipitation | HEK293 | 20022948 | |||||
(:
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|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
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| Characterization
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| Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | ||||||
| TRP channel | Interactor | ||||||||
| TRP channel | Interactor | Method | Species | Region | Species | Region | |||
| TRPC3 | IP3R1 | Co-immunoprecipitation | Human | Not determined | Rat | IP3 binding domain | 10518223 | ||
| TRPC3 | IP3R1 | Fusion protein-pull down assay | Mouse | 725-848 | Not specified | 654-688 | 11290752 | ||
(:
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:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
|
Screening
Validation: In vitro validation
Validation: In vivo validation
Characterization
Functional consequence
|
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|
| Functional consequence
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| TRP channel | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference | |||||
| TRPC3 | IP3R1 | Calcium measurement | Activation | 10518223 | |||||||
| TRPC3 | IP3R1 | Patch clamp | Activation | 10518223 | |||||||
(:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)