TRPC3 / Calmodulin
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Screening
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Experimental screening | Non-experimental screening | Reference | |||||||
TRP channel construct | Interactor source | ||||||||
TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | ||
TRPC3 | Calmodulin | Inference | Prediction | 11248050 |
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Validation: In vitro validation
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Assay with recombinant proteins | Reference | ||||||||
TRP channel construct | Interactor construct | ||||||||
TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | |
TRPC3 | Calmodulin | Fusion protein-pull down assay | Human | 1-340 | In vitro translation | Not specified | Full-length | E. coli | 12601176 |
TRPC3 | Calmodulin | Fusion protein-pull down assay | Human | 742-795 | E. coli | Rat | Full-length | In vitro translation | 11248050 |
(
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Validation: In vivo validation
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Assay with endogenous proteins | Assay with overexpressed proteins | Reference | |||||||
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | ||||||
TRP channel | Interactor | Method | Species | Region | Species | Region | |||
TRPC3 | Calmodulin | Co-immunoprecipitation | Mouse skeletal myotube lysates | 18215135 |
(
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Characterization
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Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | ||||||
TRP channel | Interactor | ||||||||
TRP channel | Interactor | Method | Species | Region | Species | Region | |||
TRPC3 | Calmodulin | Fluorescence probe labeling | Human | 764-793 | Bovine | Not determined | 100 nM | 11248050 | |
TRPC3 | Calmodulin | Fluorescence probe labeling | Human | 764-793 | Not specified | Not determined | 100 nM | 11290752 | |
TRPC3 | Calmodulin | Fusion protein-pull down assay | Human | 761-795 | Not specified | Not determined | 11290752 |
(
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Functional consequence
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TRP channel | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference | |||||
TRPC3 | Calmodulin | Patch clamp | Inhibition | 11248050 |
(
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
