TRPC1 / D2R
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Screening
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Experimental screening | Non-experimental screening | Reference | |||||||
TRP channel construct | Interactor source | ||||||||
TRP channel | Interactor | Method | Species | Region | Species | Organ/tissue | Sample type | ||
TRPC1 | D2R | Yeast two-hybrid | Not used as a bait | Human | Fetal brain | cDNA library | 18261457 |
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Validation: In vitro validation
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Assay with recombinant proteins | Reference | ||||||||
TRP channel construct | Interactor construct | ||||||||
TRP channel | Interactor | Method | Species | Region | Expression system | Species | Region | Expression system | |
TRPC1 | D2R | Fusion protein-pull down assay | Human | 638-759 | E. coli | Human | 131-151 | E. coli | 18261457 |
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Validation: In vivo validation
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Assay with endogenous proteins | Assay with overexpressed proteins | Reference | |||||||
Cell or tissue | Cell or tissue | TRP channel construct | Interactor construct | ||||||
TRP channel | Interactor | Method | Species | Region | Species | Region | |||
TRPC1 | D2R | Co-immunofluorescence staining | Monkey prefrontal cortex | 18261457 | |||||
TRPC1 | D2R | Co-immunoprecipitation | Rat striatal tissue | 18261457 | |||||
TRPC1 | D2R | Co-immunoprecipitation | Rat cortex tissue | 18261457 |
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Characterization
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Binding region mapping | Stoichiometry | Affinity (Kd) | Reference | ||||||
TRP channel | Interactor | ||||||||
TRP channel | Interactor | Method | Species | Region | Species | Region | |||
TRPC1 | D2R | Yeast two-hybrid | Human | 726-745 | Human | 131-151 | 18261457 |
(
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click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)

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Functional consequence
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TRP channel | Interactor | Method | Post-translational modification | Subcellular trafficking | Activity | Reference | |||||
TRPC1 | D2R | Cell surface biotinylation | Increase in plasma membrane level | 18261457 |
(
:
click the arrow icon to show interactions only between the corresponding TRP channel and the interactor)
